1887

Abstract

A Gram-negative, chemoheterotrophic, strictly aerobic, alkaliphilic, rod-shaped marine bacterium, designated HTCC2654, was isolated from the western Sargasso Sea by using a dilution-to-extinction culturing method. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HTCC2654 belonged to the clade of the order . The 16S rRNA gene sequence similarity of the strain with respect to other members of the r clade ranged from 90.4 to 95.1 %. In the phylogenetic analyses, the strain formed an independent phyletic line and could not be assigned to any other known genera of the . The DNA G+C content of strain HTCC2654 was 61.7 mol% by HPLC and 64.1 mol% from genome sequences. The predominant constituents of the cellular fatty acids were C 2-OH (27.3 %), 11-methyl C 7 (19.6 %) and C 7 (17.3 %), and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine, which served to differentiate the strain from other members of the clade. On the basis of the taxonomic data obtained in this study, strain HTCC2654 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is HTCC2654 (=KCCM 42376=NBRC 102057).

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2007-07-01
2019-12-16
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References

  1. Adachi, M., Kanno, T., Okamoto, R., Shinozaki, A., Fujikawa-Adachi, K. & Nishijima, T. ( 2004; ). Jannaschia cystaugens sp. nov., an Alexandrium (Dinophyceae) cyst formation-promoting bacterium from Hiroshima Bay, Japan. Int J Syst Evol Microbiol 54, 1687–1692.[CrossRef]
    [Google Scholar]
  2. Arahal, D. R., Macián, M. C., Garay, E. & Pujalte, M. J. ( 2005; ). Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 55, 2371–2376.[CrossRef]
    [Google Scholar]
  3. Biebl, H., Allgaier, M., Lunsdorf, H., Pukall, R., Tindall, B. J. & Wagner-Dobler, I. ( 2005; ). Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a. Int J Syst Evol Microbiol 55, 2377–2383.[CrossRef]
    [Google Scholar]
  4. Boettcher, K. J., Geaghan, K. K., Maloy, A. P. & Barber, B. J. ( 2005; ). Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55, 1531–1537.[CrossRef]
    [Google Scholar]
  5. Button, D. K., Schut, F., Quang, P., Martin, R. & Robertson, B. ( 1993; ). Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results. Appl Environ Microbiol 59, 881–891.
    [Google Scholar]
  6. Cho, J.-C. & Giovannoni, S. J. ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef]
    [Google Scholar]
  7. Cho, J.-C. & Giovannoni, S. J. ( 2004; ). Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol 54, 1129–1136.[CrossRef]
    [Google Scholar]
  8. Cho, J. C. & Giovannoni, S. J. ( 2006; ). Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales. Int J Syst Evol Microbiol 56, 855–859.[CrossRef]
    [Google Scholar]
  9. Choi, D. H., Yi, H., Chun, J. & Cho, B. C. ( 2006; ). Jannaschia seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. Int J Syst Evol Microbiol 56, 45–49.[CrossRef]
    [Google Scholar]
  10. Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam, S. A., McGarrell, D. M., Garrity, G. M. & Tiedje, J. M. ( 2005; ). The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 33, D294–296.
    [Google Scholar]
  11. Connon, S. A. & Giovannoni, S. J. ( 2002; ). High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl Environ Microbiol 68, 3878–3885.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  13. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  14. Giovannoni, S. & Rappé, M. ( 2000; ). Evolution, diversity and molecular ecology of marine prokaryotes. In Microbial Ecology of the Oceans, pp. 47–84. Edited by D. L. Kirchman. New York: Wiley.
  15. González, J. M., Covert, J. S., Whitman, W. B., Henriksen, J. R., Mayer, F., Scharf, B., Schmitt, R., Buchan, A., Fuhrman, J. A. & other authors ( 2003; ). Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53, 1261–1269.[CrossRef]
    [Google Scholar]
  16. Gu, J., Guo, B., Wang, Y. N., Yu, S. L., Inamori, R., Qu, R., Ye, Y. G. & Wu, X. L. ( 2007; ). Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. Int J Syst Evol Microbiol 57, 157–160.[CrossRef]
    [Google Scholar]
  17. Kovács, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703.
    [Google Scholar]
  18. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P. ( 1999; ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.[CrossRef]
    [Google Scholar]
  19. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  20. Macián, M. C., Arahal, D. R., Garay, E., Ludwig, W., Schleifer, K. H. & Pujalte, M. J. ( 2005; ). Jannaschia rubra sp. nov., a red-pigmented bacterium isolated from sea water. Int J Syst Evol Microbiol 55, 649–653.[CrossRef]
    [Google Scholar]
  21. Martens, T., Heidorn, T., Pukall, R., Simon, M., Tindall, B. J. & Brinkhoff, T. ( 2006; ). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 56, 1293–1304.[CrossRef]
    [Google Scholar]
  22. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  23. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athayle, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  24. Ostle, A. G. & Holt, J. G. ( 1982; ). Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl Environ Microbiol 44, 238–241.
    [Google Scholar]
  25. Rüger, H. J. & Höfle, M. G. ( 1992; ). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42, 133–143.[CrossRef]
    [Google Scholar]
  26. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Microbiology, pp. 611–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  28. Swofford, D. ( 2002; ). paup*: Phylogenetic analysis using parsimony (* and other methods), version 4. Sunderland, MA: Sinauer Associates.
  29. Wagner-Döbler, I., Rheims, H., Felske, A., Pukall, R. & Tindall, B. J. ( 2003; ). Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 53, 731–738.[CrossRef]
    [Google Scholar]
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Two-dimensional thin-layer chromatogram of the polar lipids of strain HTCC2654 . [PDF](33 KB)

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Transmission electron micrograph of a negatively stained cell of strain HTCC2654 . [PDF](177 KB)

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