1887

Abstract

Two bacterial strains, R2A1-13 and R2A45-3, were isolated from greenhouse soils in Korea. The cells of both strains were Gram-negative, aerobic and rod-shaped. 16S rRNA gene sequence analysis placed the isolates in the genus within the family . Strain R2A1-13 was found to be related to IAM 14301, CGMCC1.3801 and EMB47, with sequence similarities of 96.8, 95.0 and 94.6 %, respectively. Strain R2A45-3 was found to be related to EMB47 and ATCC 11947, with sequence similarities of 94.7 and 94.6 %, respectively. Both strains contained iso-C and iso-C as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone. The G+C contents of the DNA from strains R2A1-13 and R2A45-3 were 34 and 38 mol%, respectively. A polyphasic taxonomic study revealed that these strains belong to two novel species within the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains of sp. nov. and sp. nov. are R2A1-13 (=KACC 11731=DSM 18829) and R2A45-3 (=KACC 11732=DSM 18830), respectively.

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2007-07-01
2019-11-22
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References

  1. Aslam, Z., Im, W. T., Kim, M. K. & Lee, S. T. ( 2005; ). Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55, 747–751.[CrossRef]
    [Google Scholar]
  2. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M. (editors) ( 1923; ). Bergey's Manual of Determinative Bacteriology. Baltimore: Williams & Wilkins.
  3. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  5. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  6. Horn, M. A., Ihssen, J., Matthies, C., Schramm, A., Acker, G. & Drake, H. L. ( 2005; ). Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa. Int J Syst Evol Microbiol 55, 1255–1265.[CrossRef]
    [Google Scholar]
  7. Kim, B. Y., Weon, H. Y., Cousin, S., Yoo, S. H., Kwon, S. W., Go, S. J. & Stackebrandt, E. ( 2006; ). Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea. Int J Syst Evol Microbiol 56, 1645–1649.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  10. Nogi, Y., Soda, K. & Oikawa, T. ( 2005; ). Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst Appl Microbiol 28, 310–315.[CrossRef]
    [Google Scholar]
  11. Park, M., Lu, S., Ryu, S. H., Chung, B. S., Park, W., Kim, C.-J. & Jeon, C. O. ( 2006; ). Flavobacterium croceum sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56, 2443–2447.[CrossRef]
    [Google Scholar]
  12. Saha, P. & Chakrabarti, T. ( 2006; ). Flavobacterium indicum sp. nov., isolated from warm spring water in Assam, India. Int J Syst Evol Microbiol 56, 2617–2621.[CrossRef]
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  15. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swing, J. ( 2005; ). Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55, 769–772.[CrossRef]
    [Google Scholar]
  16. Wang, Z. W., Liu, Y. H., Dai, X., Wang, B. J., Jiang, C. Y. & Liu, S. J. ( 2006; ). Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56, 439–442.[CrossRef]
    [Google Scholar]
  17. Xie, C. H. & Yokota, A. ( 2006; ). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 1117–1121.[CrossRef]
    [Google Scholar]
  18. Yi, H. & Chun, J. ( 2006; ). Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int J Syst Evol Microbiol 56, 1239–1244.[CrossRef]
    [Google Scholar]
  19. Yi, H., Oh, H. M., Lee, J. H., Kim, S. J. & Chun, J. ( 2005; ). Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55, 637–641.[CrossRef]
    [Google Scholar]
  20. Yoon, J. H., Kang, S. J. & Oh, T. K. ( 2006; ). Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 997–1000.[CrossRef]
    [Google Scholar]
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