1887

Abstract

Two bacterial strains, R2A1-13 and R2A45-3, were isolated from greenhouse soils in Korea. The cells of both strains were Gram-negative, aerobic and rod-shaped. 16S rRNA gene sequence analysis placed the isolates in the genus within the family . Strain R2A1-13 was found to be related to IAM 14301, CGMCC1.3801 and EMB47, with sequence similarities of 96.8, 95.0 and 94.6 %, respectively. Strain R2A45-3 was found to be related to EMB47 and ATCC 11947, with sequence similarities of 94.7 and 94.6 %, respectively. Both strains contained iso-C and iso-C as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone. The G+C contents of the DNA from strains R2A1-13 and R2A45-3 were 34 and 38 mol%, respectively. A polyphasic taxonomic study revealed that these strains belong to two novel species within the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains of sp. nov. and sp. nov. are R2A1-13 (=KACC 11731=DSM 18829) and R2A45-3 (=KACC 11732=DSM 18830), respectively.

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2007-07-01
2020-09-22
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References

  1. Aslam Z., Im W. T., Kim M. K., Lee S. T. 2005; Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55:747–751 [CrossRef]
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. (editors) 1923 Bergey's Manual of Determinative Bacteriology Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  5. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  6. Horn M. A., Ihssen J., Matthies C., Schramm A., Acker G., Drake H. L. 2005; Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa . Int J Syst Evol Microbiol 55:1255–1265 [CrossRef]
    [Google Scholar]
  7. Kim B. Y., Weon H. Y., Cousin S., Yoo S. H., Kwon S. W., Go S. J., Stackebrandt E. 2006 Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea. Int J Syst Evol Microbiol 56, 1645–1649 [CrossRef]
  8. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  10. Nogi Y., Soda K., Oikawa T. 2005; Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst Appl Microbiol 28:310–315 [CrossRef]
    [Google Scholar]
  11. Park M., Lu S., Ryu S. H., Chung B. S., Park W., Kim C.-J., Jeon C. O. 2006; Flavobacterium croceum sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56:2443–2447 [CrossRef]
    [Google Scholar]
  12. Saha P., Chakrabarti T. 2006; Flavobacterium indicum sp. nov., isolated from warm spring water in Assam, India. Int J Syst Evol Microbiol 56:2617–2621 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  15. Van Trappen S., Vandecandelaere I., Mergaert J., Swing J. 2005; Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55:769–772 [CrossRef]
    [Google Scholar]
  16. Wang Z. W., Liu Y. H., Dai X., Wang B. J., Jiang C. Y., Liu S. J. 2006; Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56:439–442 [CrossRef]
    [Google Scholar]
  17. Xie C. H., Yokota A. 2006; Reclassification of [ Flavobacterium ] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1117–1121 [CrossRef]
    [Google Scholar]
  18. Yi H., Chun J. 2006; Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int J Syst Evol Microbiol 56:1239–1244 [CrossRef]
    [Google Scholar]
  19. Yi H., Oh H. M., Lee J. H., Kim S. J., Chun J. 2005; Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55:637–641 [CrossRef]
    [Google Scholar]
  20. Yoon J. H., Kang S. J., Oh T. K. 2006; Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:997–1000 [CrossRef]
    [Google Scholar]
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