1887

Abstract

A Gram-negative, rod-shaped bacterial strain, designated K19414, was isolated from a chironomid (Diptera; Chironomidae) egg mass which was sampled from Kishon River in northern Israel. Phylogenetic analysis based on the 16S rRNA gene sequence positioned the novel strain among the genus , with closest similarity to KMM 1406. The levels of similarity to type strains of species were lower than 96.5 %. Isolate K19414 is aerobic, motile by means of a single polar flagellum, catalase-negative and oxidase-positive; growth was observed at salinities of 0–2 % NaCl and the temperature for growth ranged from 4 to 40 °C. The major cellular fatty acids are 16 : 0 (14.8 %) and 16 : 17 and/or 15 : 0 iso 2-OH (25.76 %). The DNA G+C content is 49.9 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain K19414 (=LMG 23818 =DSM 18694) was classified in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64927-0
2007-08-01
2021-03-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/8/1872.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64927-0&mimeType=html&fmt=ahah

References

  1. Brettar I., Christen R., Höfle M. G. 2002; Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52:1851–1857 [CrossRef]
    [Google Scholar]
  2. Brettar I., Christen R., Höfle M. G. 2006; Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56:2177–2183 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  4. Felske A., Rheims H., Wolterink A., Stackebrandt E., Akkermans A. D. L. 1997; Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. Microbiology 143:2983–2989 [CrossRef]
    [Google Scholar]
  5. Halpern M., Landsberg O., Raats D., Rosenberg E. 2007; Culturable and VBNC Vibrio cholerae : interactions with chironomid egg masses and their bacterial population. Microb Ecol 53:285–293 [CrossRef]
    [Google Scholar]
  6. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  7. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  8. Raats D., Halpern M. 2007; Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. Int J Syst Evol Microbiol 57:255–259 [CrossRef]
    [Google Scholar]
  9. Romanenko L. A., Uchino M., Falsen E., Zhukova N. V., Mikhailov V. V., Uchimura T. 2003; Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 53:1973–1977 [CrossRef]
    [Google Scholar]
  10. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  11. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2.4.1−2.4.5 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Smith J. A, Struhl K. New York: Greene Publishing and Wiley-Interscience;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64927-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64927-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error