1887

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Volume 57, Issue 8

sp. nov., a novel strictly anaerobic, Gram-negative, hemicellulose-decomposing bacterium isolated from plant residue and rice roots in irrigated rice-field soil Free

Abstract

Two strictly anaerobic bacterial strains, KB7 and A42, were isolated from rice plant residue and living rice roots, respectively, from irrigated rice-field soil in Japan. These two strains were closely related to each other with 16S rRNA gene sequence similarity of 99.8 %. Both strains showed almost the same physiological properties. Cells were Gram-negative, non-motile, non-spore-forming rods. Growth was remarkably stimulated by the addition of haemin to the medium. The strains utilized various saccharides including xylan, xylose, pectin and carboxymethylcellulose and produced acetate and succinate with small amounts of formate and malate. The strains grew at 10–40 °C; optimum growth was observed at 30 °C and pH 5.7–6.7. Oxidase, catalase and nitrate-reducing activities were not detected. Aesculin was hydrolysed. The major cellular fatty acids were anteiso-C, iso-C, C and iso-C 3-OH. Menaquinones MK-11 and MK-11(H) were the major respiratory quinones and the genomic DNA G+C content was 39.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed both strains in the phylum . 16S rRNA gene sequence analysis showed that the most related species to both strains was (92.8–92.9 % similarity). and were the next most closely related known species with sequence similarities of 91.9–92.4 %. Based on differences in the phylogenetic, ecological, physiological and chemotaxonomic characteristics between the two isolates and related species, it is proposed that strains KB7 and A42 represent a novel species, sp. nov. This is the first described species derived from a natural habitat; all other species are from mammalian sources. The type strain of is KB7 (=JCM 13650=DSM 17968).

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Keyword(s): CFA, whole-cell fatty acid and CMC, carboxymethylcellulose
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2007-08-01
2024-04-26
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Prevotella paludivivens sp. nov., a novel strictly anaerobic, Gram-negative, hemicellulose-decomposing bacterium isolated from plant residue and rice roots in irrigated rice-field soil
Int J Syst Evol Microbiol 57, 1803 (2007); https://doi.org/10.1099/ijs.0.64914-0
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