1887

Abstract

A bacterial strain designated MACL04 was isolated from Lake Martel, a subterraneous saline lake in Mallorca (Spain). The complete 16S rRNA gene sequence of this strain showed nearly 100 % similarity to that of B-5. Despite this high similarity, strain MACL04 showed phenotypic, chemotaxonomic and molecular differences with respect to , indicating that strain MACL04 represents a separate species. Cells of strain MACL04 were motile by means of a single polar or subpolar flagellum and colonies formed on media containing 1 % (v/v) Tween 20 were opaque and mucoid, with blue–green iridescence. The generation time of strain MACL04 in this medium was approximately half that of B-5 and strain MACL04 did not produce lipases after incubation for 5 days. Strain MACL04 did not require NaCl for growth and grew in the presence of up to 15 % (w/v) NaCl. The strain was able to use alkanes as a sole carbon source; however, glucose could also be used, albeit weakly, as a carbon source. Several amino acids and organic acids were used as carbon sources. Strain MACL04 produced acid in media containing pyruvate as the sole carbon source. The major fatty acids were C cyclo 8 and C. The fatty acid C 8, present in strain MACL04, was not detected in the recognized species. The sequences of the large and short 16S–23S intergenic spacer regions showed similarities of 97.2 and 98.8 % (ungapped) with respect to B-5. Partial sequences of and genes showed 94.0 % similarity between strain MACL04 and B-5. The G+C content of strain MACL04 was 62.8 mol%. The data from this polyphasic study indicate that strain MACL04 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MACL04 (=LMG 22508=CECT 5683).

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2007-06-01
2020-01-23
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Colony morphologies of strain MACL04 and B-5 on media containing Tween 20. [PDF](30 KB)

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Electron micrograph of a cell of strain MACL04 . [PDF](117 KB)

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TP-RAPD profiles of strain MACL04 and recognized species of the genus . [PDF](134 KB)

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Neighbour-joining phylogenetic tree based on 16S-23S rRNA gene intergenic spacer sequences of strain MACL04 and phylogenetically related species of the genus . [PDF](15 KB)

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Neighbour-joining phylogenetic tree based on partial sequences of strain MACL04 and phylogenetically related species of the genus . [PDF](15 KB)

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Neighbour-joining phylogenetic tree based on partial sequences of strain MACL04 and phylogenetically related species of the genus . [PDF](15 KB)

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Fatty acid profiles of and recognized species of the genus . [PDF](46 KB)

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