1887

Abstract

The taxonomic status of a bacterial isolate from the sludge of a wastewater treatment plant was characterized by using a polyphasic taxonomic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genus , fatty acids C and C (found by pyrolysis gas chromatography) and a dihydrogenated menaquinone with nine isoprene units [MK-9(H2)] as the predominant menaquinone. The genus assignment was confirmed by 16S rRNA gene sequencing. Comparative analysis of the 16S rRNA gene sequence showed that the novel isolate constitutes a hitherto unknown subline within the genus , displaying 95.9 to 97.6 % gene sequence similarity to the recognized species of the genus. The novel isolate was distinguished from the type strains of phylogenetically related species by using a set of phenotypic features. The genotypic and phenotypic data show that the new strain merits classification as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMMIB WWCC-22 (=DSM 45064=CCUG 53555).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64893-0
2007-05-01
2020-09-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/1065.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64893-0&mimeType=html&fmt=ahah

References

  1. Becker B., Lechevalier M. P., Gordon R. E., Lechevalier H. A. 1964; Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysates. Appl Microbiol 12:421–423
    [Google Scholar]
  2. Bendinger B., Rainey F. A., Kroppenstedt R. M., Moormann M., Klatte S. 1995; Gordona hydrophobica sp. nov., isolated from biofilters for waste gas treatment. Int J Syst Bacteriol 45:544–548 [CrossRef]
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Gordon R. E. 1966; Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45:355–364 [CrossRef]
    [Google Scholar]
  9. Gordon R. E. 1967; The taxonomy of soil bacteria. In The Ecology of Soil Bacteria pp  293–321 Edited by Gray T. R. G., Parkinson B. Liverpool: University Press;
    [Google Scholar]
  10. Gordon R. E., Mihm J. M. 1957; A comparative study of some strains received as nocardiae. J Bacteriol 73:15–27
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Kageyama A., Iida S., Yazawa K., Kudo T., Suzuki S.-i., Koga T., Saito H., Inagawa H., Wada A. other authors 2006 Gordonia araii sp. nov. and Gordonia effusa sp nov., isolated from patients in Japan. Int J Syst Evol Microbiol 56, 1817–1821 [CrossRef]
  13. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M. 2000; Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 50:2031–2036 [CrossRef]
    [Google Scholar]
  14. Kim K. K., Lee C. S., Kroppenstedt R. M., Stackebrandt E., Lee S. T. 2003; Gordonia sihwensis sp. nov., a novel nitrate-reducing bacterium isolated from a wastewater-treatment bioreactor. Int J Syst Evol Microbiol 53:1427–1433 [CrossRef]
    [Google Scholar]
  15. Klatte S., Kroppenstedt R. M., Schumann P., Altendorf K.-H., Rainey F. A. 1996; Gordona hirsuta sp. nov. Int J Syst Bacteriol 46:876–880 [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P. 1968; Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71:934–944
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  18. Lechevalier M. P., de Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  19. Linos A., Berekaa M. M., Steinbüchel A., Kim K. K., Spröer C., Kroppenstedt R. M. 2002; Gordonia westfalica sp. nov., a novel rubber-degrading actinomycete. Int J Syst Evol Microbiol 52:1133–1139 [CrossRef]
    [Google Scholar]
  20. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–223 [CrossRef]
    [Google Scholar]
  22. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Soddell J. A., Stainsby F. M., Eales K. L., Seviour R. J., Goodfellow M. 2006; Gordonia defluvii sp. nov., an actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 56:2265–2269 [CrossRef]
    [Google Scholar]
  25. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  26. Tsukamura M. 1971; Proposal of a new genus, Gordona , for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J Gen Microbiol 68:15–26 [CrossRef]
    [Google Scholar]
  27. Tsukamura M. 1978; Numerical classification of Rhodococcus (formerly Gordona ) organisms recently isolated from sputa of patients: description of Rhodococcus sputi Tsukamura sp. nov. Int J Syst Bacteriol 28:169–181 [CrossRef]
    [Google Scholar]
  28. Tsukamura M. 1982; Numerical analysis of the taxonomy of Nocardiae and Rhodococci . Microbiol Immunol 26:1101–1119 [CrossRef]
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  30. Yassin A. F. 1988; Chemotaxonomische Untersuchungen zur vereinfachten Differenzierung und Identifizierung von aeroben Aktinomyzeten und Mykobakterien . Inaaugural-Dissertation zur erlangung des Doktorgrades der Mathematische-Naturwissenschaftlichen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn (in German
  31. Yassin A. F., Hupfer H. 2006; Williamsia deligens sp. nov., isolated from human blood. Int J Syst Evol Microbiol 56:193–197 [CrossRef]
    [Google Scholar]
  32. Yassin A. F., Binder C., Schaal K. P. 1993a; Identification of mycobacterial isolates by thin-layer and capillary gas-liquid chromatography under diagnostic routine conditions. Zentralbl Bakteriol 278:34–48 [CrossRef]
    [Google Scholar]
  33. Yassin A. F., Haggenei B., Budzikiewicz H., Schaal K. P. 1993b; Fatty acid and polar lipid composition of the genus Amycolatopsis : application of fast atom bombardment mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43:414–420 [CrossRef]
    [Google Scholar]
  34. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Lee H. L., Schaal K. P. 1995; Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45:522–527 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64893-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64893-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error