1887

Abstract

The taxonomic status of a bacterial isolate from the sludge of a wastewater treatment plant was characterized by using a polyphasic taxonomic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genus , fatty acids C and C (found by pyrolysis gas chromatography) and a dihydrogenated menaquinone with nine isoprene units [MK-9(H2)] as the predominant menaquinone. The genus assignment was confirmed by 16S rRNA gene sequencing. Comparative analysis of the 16S rRNA gene sequence showed that the novel isolate constitutes a hitherto unknown subline within the genus , displaying 95.9 to 97.6 % gene sequence similarity to the recognized species of the genus. The novel isolate was distinguished from the type strains of phylogenetically related species by using a set of phenotypic features. The genotypic and phenotypic data show that the new strain merits classification as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMMIB WWCC-22 (=DSM 45064=CCUG 53555).

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2007-05-01
2019-12-15
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References

  1. Becker, B., Lechevalier, M. P., Gordon, R. E. & Lechevalier, H. A. ( 1964; ). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysates. Appl Microbiol 12, 421–423.
    [Google Scholar]
  2. Bendinger, B., Rainey, F. A., Kroppenstedt, R. M., Moormann, M. & Klatte, S. ( 1995; ). Gordona hydrophobica sp. nov., isolated from biofilters for waste gas treatment. Int J Syst Bacteriol 45, 544–548.[CrossRef]
    [Google Scholar]
  3. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  8. Gordon, R. E. ( 1966; ). Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45, 355–364.[CrossRef]
    [Google Scholar]
  9. Gordon, R. E. ( 1967; ). The taxonomy of soil bacteria. In The Ecology of Soil Bacteria, pp. 293–321. Edited by T. R. G. Gray & B. Parkinson. Liverpool: University Press.
  10. Gordon, R. E. & Mihm, J. M. ( 1957; ). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27.
    [Google Scholar]
  11. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  12. Kageyama, A., Iida, S., Yazawa, K., Kudo, T., Suzuki, S.-i., Koga, T., Saito, H., Inagawa, H., Wada, A. & other authors ( 2006; ). Gordonia araii sp. nov. and Gordonia effusa sp. nov., isolated from patients in Japan. Int J Syst Evol Microbiol 56, 1817–1821.[CrossRef]
    [Google Scholar]
  13. Kim, S. B., Brown, R., Oldfield, C., Gilbert, S. C., Iliarionov, S. & Goodfellow, M. ( 2000; ). Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 50, 2031–2036.[CrossRef]
    [Google Scholar]
  14. Kim, K. K., Lee, C. S., Kroppenstedt, R. M., Stackebrandt, E. & Lee, S. T. ( 2003; ). Gordonia sihwensis sp. nov., a novel nitrate-reducing bacterium isolated from a wastewater-treatment bioreactor. Int J Syst Evol Microbiol 53, 1427–1433.[CrossRef]
    [Google Scholar]
  15. Klatte, S., Kroppenstedt, R. M., Schumann, P., Altendorf, K.-H. & Rainey, F. A. ( 1996; ). Gordona hirsuta sp. nov. Int J Syst Bacteriol 46, 876–880.[CrossRef]
    [Google Scholar]
  16. Lechevalier, M. P. ( 1968; ). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944.
    [Google Scholar]
  17. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  18. Lechevalier, M. P., de Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  19. Linos, A., Berekaa, M. M., Steinbüchel, A., Kim, K. K., Spröer, C. & Kroppenstedt, R. M. ( 2002; ). Gordonia westfalica sp. nov., a novel rubber-degrading actinomycete. Int J Syst Evol Microbiol 52, 1133–1139.[CrossRef]
    [Google Scholar]
  20. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  21. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–223.[CrossRef]
    [Google Scholar]
  22. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Soddell, J. A., Stainsby, F. M., Eales, K. L., Seviour, R. J. & Goodfellow, M. ( 2006; ). Gordonia defluvii sp. nov., an actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 56, 2265–2269.[CrossRef]
    [Google Scholar]
  25. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  26. Tsukamura, M. ( 1971; ). Proposal of a new genus, Gordona, for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J Gen Microbiol 68, 15–26.[CrossRef]
    [Google Scholar]
  27. Tsukamura, M. ( 1978; ). Numerical classification of Rhodococcus (formerly Gordona) organisms recently isolated from sputa of patients: description of Rhodococcus sputi Tsukamura sp. nov. Int J Syst Bacteriol 28, 169–181.[CrossRef]
    [Google Scholar]
  28. Tsukamura, M. ( 1982; ). Numerical analysis of the taxonomy of Nocardiae and Rhodococci. Microbiol Immunol 26, 1101–1119.[CrossRef]
    [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  30. Yassin, A. F. ( 1988; ). Chemotaxonomische Untersuchungen zur vereinfachten Differenzierung und Identifizierung von aeroben Aktinomyzeten und Mykobakterien. Inaaugural-Dissertation zur erlangung des Doktorgrades der Mathematische-Naturwissenschaftlichen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn (in German).
  31. Yassin, A. F. & Hupfer, H. ( 2006; ). Williamsia deligens sp. nov., isolated from human blood. Int J Syst Evol Microbiol 56, 193–197.[CrossRef]
    [Google Scholar]
  32. Yassin, A. F., Binder, C. & Schaal, K. P. ( 1993a; ). Identification of mycobacterial isolates by thin-layer and capillary gas-liquid chromatography under diagnostic routine conditions. Zentralbl Bakteriol 278, 34–48.[CrossRef]
    [Google Scholar]
  33. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P. ( 1993b; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef]
    [Google Scholar]
  34. Yassin, A. F., Rainey, F. A., Brzezinka, H., Burghardt, J., Lee, H. L. & Schaal, K. P. ( 1995; ). Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45, 522–527.[CrossRef]
    [Google Scholar]
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