1887

Abstract

A strictly aerobic, Gram-negative, gliding, dull-orange-pigmented, rod-shaped bacterium, designated strain Shu-F-UV2-2, was isolated from sediment (Carp Island, Republic of Palau) and was the focus of a polyphasic taxonomic study. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated to the family ‘’ of the phylum and that it showed highest sequence similarity (85.5 %) to NBRC 100898. The novel isolate could be differentiated phenotypically and physiologically from recognized members of the family ‘’. The G+C content of the DNA was 43.0 mol%, MK-7 was the major menaquinone and iso-C, C 7 and C 5 were the major fatty acids. On the basis of this polyphasic evidence, it was concluded that strain Shu-F-UV2-2 represents a novel species in a new genus of the family ‘’, for which the name gen. nov., sp. nov. is proposed. The type strain is Shu-F-UV2-2 (=MBIC06993=IAM 15413=KCTC 12867).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64845-0
2007-05-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/964.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64845-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P., McCammon S. A., Brown M. V., Nichols D. S., McMeekin T. A. 1997; Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63:3068–3078
    [Google Scholar]
  2. Cottrell M. T., Kirchman D. L. 2000; Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol 66:5116–5122 [CrossRef]
    [Google Scholar]
  3. DeLong E. F., Franks D. G., Alldredge A. L. 1993; Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol Oceanogr 38:924–934 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Glöckner F. O., Fuchs B. M., Amann R. 1999; Bacterioplankton composition of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65:3721–3726
    [Google Scholar]
  6. Hudson J. A., Schofield K. M., Morgan H. W., Daniel R. M. 1989; Thermonema lapsum gen. nov., sp. nov. a thermophilic gliding bacteria. Int J Syst Bacteriol 39:485–487 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  9. Lewin R. A. 1970 Flexithrix dorotheae gen. et sp. nov. (Flexibacterales); and suggestions for reclassifying sheathed bacteria. Can J Microbiol 16511–515 [CrossRef]
  10. Ludwig W., Klenk H.-P. 2001; Overview: a phylogenetic backbone and taxonomic framework for prokaryotic systematics. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  49–65 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  11. Lyman J., Fleming R. H. 1940; Composition of sea water. J Mar Res 3:134–146
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp  21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Nakagawa Y., Hamana K., Sakane T., Yamasato K. 1997 Reclassification of Cytophaga aprica (Lewin 1969) Reichenbach 1989 in Flammeovirga gen. nov. as Flammeovirga aprica comb.nov. and of Cytophaga diffuens (ex Stanier 1940; emend. Lewin 1969) Reichenbach 1989 in Persicobacter gen. nov. as Persicobacter diffuens comb. nov. Int J Syst Bacteriol 47, 220–223 [CrossRef]
  16. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Reichenbachia agariperforans gen. nov., sp. nov. a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides . Int J Syst Evol Microbiol 53:81–85 [CrossRef]
    [Google Scholar]
  17. O'Sullivan L. A., Weightman A. J., Fry J. C. 2002; New degenerate Cytophaga–Flexibacter–Bacteroides -specific 16S ribosomal DNA-targeted oligonucleotide probes reveal high bacterial diversity in River Taff epilithon. Appl Environ Microbiol 68:201–210 [CrossRef]
    [Google Scholar]
  18. O'Sullivan L. A., Fuller K. E., Thomas E. M., Turley C. M., Fry J. C., Weightman A. J. 2004; Distribution and culturability of the uncultivated ‘AGG58 cluster’ of the Bacteroidetes phylum in aquatic environments. FEMS Microbiol Ecol 47:359–370 [CrossRef]
    [Google Scholar]
  19. Perry L. B. 1973; Gliding motility in some non-spreading flexibacteria. J Appl Bacteriol 36:227–232 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Takahashi M., Suzuki K., Nakagawa Y. 2006; Emendation of the genus Flammeovirga and Flammeovirga aprica with the proposal of Flammeovirga arenaria nom. rev., comb. nov. and Flammeovirga yaeyamensis sp. nov.. Int J Syst Evol Microbiol 562095–2100 [CrossRef]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  23. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  24. Xie C., Yokota A. 2003; Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64845-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64845-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error