1887

Abstract

A polyphasic taxonomic study of the lactic acid bacteria (LAB) population in three traditional Belgian sourdoughs, sampled between 2002 and 2004, revealed a group of isolates that could not be assigned to any recognized LAB species. Initially, sourdough isolates were screened by means of (GTG)-PCR fingerprinting. Four isolates displaying unique (GTG)-PCR patterns were further investigated by means of phenylalanyl-tRNA synthase () gene sequence analysis and represented a bifurcated branch that could not be allocated to any LAB species present in the in-house database. Their phylogenetic affiliation was determined using 16S rRNA gene sequence analysis and showed that the four sourdough isolates belong to the group with , and as closest relatives. Further genotypic and phenotypic studies, including whole-cell protein analysis (SDS-PAGE), amplified fragment length polymorphism (AFLP) fingerprinting, DNA–DNA hybridization, DNA G+C content analysis, growth characteristics and biochemical features, demonstrated that the new sourdough isolates represent a novel species for which the name sp. nov. is proposed. The type strain of the new species is LMG 23699 (=CCUG 53174).

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2007-07-01
2020-09-23
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References

  1. Cooper J. E., Feil E. J. 2004; Multilocus sequence typing – what is resolved?. Trends Microbiol 12:373–377 [CrossRef]
    [Google Scholar]
  2. de Man J. C., Rogosa M., Scharpe E. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135 [CrossRef]
    [Google Scholar]
  3. De Vuyst L., Neysens P. 2005; The sourdough microflora: biodiversity and metabolic interactions. Trends Food Sci Technol 16:43–56 [CrossRef]
    [Google Scholar]
  4. Ehrmann M. A., Müller M. R. A., Vogel R. F. 2003; Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov. Int J Syst Evol Microbiol 53:7–13 [CrossRef]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Gevers D., Huys G., Swings J. 2001; Applicability of rep-PCR fingerprinting for differentiation of Lactobacillus species. FEMS Microbiol Lett 205:31–36 [CrossRef]
    [Google Scholar]
  7. Gobbetti M. 1998; The sourdough microflora: interactions of lactic acid bacteria and yeasts. Trends Food Sci Technol 9:267–274 [CrossRef]
    [Google Scholar]
  8. Hammes W. P., Gänzle M. G. 1998; Sourdough breads and related products. In Microbiology of Fermented Foods pp  199–216 Edited by Wood B. J. B. London: Blackie;
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  10. Meroth C. B., Walter J., Hertel C., Brandt M. J., Hammes W. P. 2003; Monitoring the bacterial population dynamics in sourdough fermentation processes by using PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol 69:475–482 [CrossRef]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. 2005; Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151:2141–2150 [CrossRef]
    [Google Scholar]
  13. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  14. Reuter G. 1983; Lactobacillus alimentarius sp. nov., nom. rev. and Lactobacillus farciminis sp. nov., nom. rev.. Syst Appl Microbiol 4277–279 [CrossRef]
    [Google Scholar]
  15. Rossello-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
    [Google Scholar]
  16. Scheirlinck I., Van der Meulen R., Van Schoor A., Cleenwerck I., Huys G., Vandamme P., De Vuyst L., Vancanneyt M. 2007; Lactobacillus namurensis sp. nov., isolated from a traditional Belgian sourdough. Int J Syst Evol Microbiol 57:223–227 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; A place for DNA–DNA reassociation and 16S ribosomal RNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. 2001; Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24:520–538 [CrossRef]
    [Google Scholar]
  19. Valcheva R., Ferchichi M. F., Korakli M., Ivanova I., Gänzle M. G., Vogel R. F., Prevost H., Onno B., Dousset X. 2006; Lactobacillus nantensis sp. nov., isolated from French wheat sourdough. Int J Syst Evol Microbiol 56:587–591 [CrossRef]
    [Google Scholar]
  20. Vancanneyt M., Naser S. M., Engelbeen K., De Wachter M., Van der Meulen R., Cleenwerck I., Hoste B., De Vuyst L., Swings J. 2006; Reclassification of Lactobacillus brevis strains LMG 11494 and LMG 11984 as Lactobacillus parabrevis sp. nov. Int J Syst Evol Microbiol 56:1553–1557 [CrossRef]
    [Google Scholar]
  21. Versalovic J., Schneider M., De Bruijn F. J., Lupski J. R. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40
    [Google Scholar]
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