1887

Abstract

A polyphasic taxonomic study of the lactic acid bacteria (LAB) population in three traditional Belgian sourdoughs, sampled between 2002 and 2004, revealed a group of isolates that could not be assigned to any recognized LAB species. Initially, sourdough isolates were screened by means of (GTG)-PCR fingerprinting. Four isolates displaying unique (GTG)-PCR patterns were further investigated by means of phenylalanyl-tRNA synthase () gene sequence analysis and represented a bifurcated branch that could not be allocated to any LAB species present in the in-house database. Their phylogenetic affiliation was determined using 16S rRNA gene sequence analysis and showed that the four sourdough isolates belong to the group with , and as closest relatives. Further genotypic and phenotypic studies, including whole-cell protein analysis (SDS-PAGE), amplified fragment length polymorphism (AFLP) fingerprinting, DNA–DNA hybridization, DNA G+C content analysis, growth characteristics and biochemical features, demonstrated that the new sourdough isolates represent a novel species for which the name sp. nov. is proposed. The type strain of the new species is LMG 23699 (=CCUG 53174).

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2007-07-01
2019-10-23
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vol. , part 7, pp. 1461-1467

Cluster analysis of digitized protein profiles of and closest phylogenetic relatives. The corresponding dendrogram was constructed derived from UPGMA cluster analysis (correlation coefficients for convenience expressed as percentage).



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