1887

Abstract

A yellow-pigmented, Gram-positive, aerobic, non-motile, non-spore-forming, irregular rod-shaped bacterium (strain TAN 31504) was isolated from the bacteriophagous nematode . Based on 16S rRNA gene sequence similarity, DNA G+C content of 69.5 mol%, 2,4-diaminobutyric acid in the cell-wall peptidoglycan, major menaquinone MK-11, abundance of anteiso- and iso-fatty acids, polar lipids diphosphatidylglycerol and phosphatidylglycerol and a number of shared biochemical characteristics, strain TAN 31504 was placed in the genus . DNA–DNA hybridization comparisons demonstrated a 91 % DNA–DNA relatedness between strain TAN 31504 and LMG 22506 indicating that these two strains belong to the same species, when the recommended threshold value of 70 % DNA–DNA relatedness for the definition of a bacterial species by the ad hoc committee on reconciliation of approaches to bacterial systematics is considered. Based on distinct differences in morphology, physiology, chemotaxonomic markers and various biochemical characteristics, it is proposed to split the species into two novel subspecies, subsp. subsp. nov. (type strain L-1=CIP 108389=LMG 22506) and subsp. subsp. nov. (type strain TAN 31504=DSM 18340=ATCC BAA-1336).

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2007-12-01
2020-01-22
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Transmission electron micrographs of cells of strain TAN 31504 ( subsp. subsp. nov.). [PDF](642 KB)

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Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the relationship of strain TAN 31504 ( subsp. subsp. nov.) to members of the genus and other reference organisms. [PDF](19 KB)

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