1887

Abstract

A slightly pink-coloured strain, strain DFL-11, was isolated from single cells of the marine dinoflagellate and was found to contain the genes encoding two proteins of the photosynthetic reaction centre, and . 16S rRNA gene sequence analysis revealed that the novel strain belonged to the -2 subgroup of the and was most closely related to (97.7 % similarity), (98.0 %) and (98.0 %). Dark-grown cells of strain DFL-11 contained small amounts of bacteriochlorophyll (bchl ) and a carotenoid. Cells of strain DFL-11 were rods, 0.5–0.7×0.9–3.0 μm in size and motile by means of a single, subpolarly inserted flagellum. The novel strain was strictly aerobic and utilized a wide range of organic carbon sources, including fatty acids, tricarboxylic acid cycle intermediates and sugars. Biotin and thiamine were required as growth factors. Growth was obtained at sea salt concentrations of between 1 and 10 % (w/v), at a pH between 6 and 9.2 and at a temperature of up to 33 °C (optimum, 26 °C). Nitrate was not reduced and indole was not produced from tryptophan. Strain DFL11 was resistant to potassium tellurite and transformed it to elemental tellurium. The major respiratory lipoquinone was ubiquinone 10 (Q10). The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, an unidentified aminolipid and the glycolipid sulphoquinovosyldiacylglyceride. The fatty acids comprised 16 : 17, 16 : 0, 18 : 17, 18 : 0, 11-methyl 18 : 16, 11-methyl 20 : 16, 20 : 17, 22 : 0, 22 : 1 and the hydroxy fatty acids 3-OH 14 : 0, 3-OH 16 : 0 (ester-linked), 3-OH 18 : 0, 3-OH 20 : 1 and 3-OH 20 : 0, all of which are amide-linked. The DNA G+C value was 56 mol%. Comparative analysis of -2 subgroup 16S rRNA gene sequences showed that the type species of the genus , , is only distantly related to (95.3 % sequence similarity). Based on the combination of the 16S rRNA gene sequence data, a detailed chemotaxonomic study and the biochemical and physiological properties of members of the genera , and , it is proposed that , , are transferred to a new genus, gen. nov., as comb. nov., comb. nov. and comb. nov. The type species of the new genus is sp. nov., with strain DFL-11 (=DSM 17067=NCIMB 14079) as the type strain. The genes of the photosynthesis reaction centre were shown to be present in some, but not all, species of the new genus and they were identified for the first time in . In accordance with the new data collected in this study, emended descriptions are provided for the genera , and .

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2007-05-01
2019-10-18
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An electron micrograph showing flagellar insertion in cells of DFL-11 . [PDF](113 KB)

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A similarity matrix of 16S rRNA gene sequences for DFL-11 and related taxa. [PDF](36 KB)

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