1887

Abstract

A Gram-negative, short rod to oval-shaped bacterium (strain MW2a) was isolated from the surface of leaves of the black mangrove and subjected to a polyphasic taxonomic study. Strain MW2a was moderately halophilic, growing at NaCl concentrations in the range 0–25 % (w/v) with optimum growth at 5 % (w/v) NaCl. Growth occurred at 12–40 °C (optimum, 30–35 °C) and at pH 5.0–9.0 (optimum, pH 7.0–8.0). Strain MW2a was strictly aerobic. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus . The closest relative was , with 98.6 % 16S rRNA gene sequence similarity. The DNA G+C content of strain MW2a was 61.5 mol%, which is in the range of values for species. DNA–DNA hybridization with showed a relatedness of 42 % and lower values were obtained with respect to other related species. The major fatty acids were C, C cyclo 8, C 7 and C 3-OH. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MW2a represents a novel species within the genus . The name sp. nov. is proposed, with strain MW2a (=CECT 7193=CCM 7396) as the type strain.

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2007-05-01
2020-01-23
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