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Abstract

A novel yellow-pigmented, non-motile, non-sporulating, catalase- and oxidase-positive, obligately aerobic, moderately halophilic, facultatively alkaliphilic Gram-positive coccus, strain YIM Y15, was isolated from a brine sample from a salt mine in Yunnan, south-west China. Strain YIM Y15 grew in the presence of 0.5–25 % (w/v) NaCl and at pH 6.0–10.0, with optimum growth at 8–10 % (w/v) NaCl and pH 8.0. It grew at 4–45 °C, with optimum growth at 37.0 °C. The major cellular fatty acids were anteiso-C and iso-C. Menaquinone 6 (MK-6) was the major respiratory quinone. The cell wall contained Lys and Gly. The DNA G+C content was 46.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that strain YIM Y15 was a member of the genus , with low 16S rRNA gene sequence similarity to the type strains of the five described species of the genus, JCM 11311 (sequence similarity 96.0 %), DSM 5351 (94.9 %), KCTC 13030 (94.8 %), LMG 22840 (94.8 %) and DSM 5352 (94.7 %). Together with the phenotypic differences, these results supported the proposal of a novel species of the genus , sp. nov., with YIM Y15 (=DSM 17847 =CGMCC 1.6302) as the type strain.

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2007-10-01
2020-01-25
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