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A novel thermophilic and heterotrophic sulfate-reducing bacterium, strain TFISO9T, was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV in the Southern Okinawa Trough. The cells were motile rods 2.5–5.0 μm in length and 0.6–0.9 μm in width. Strain TFISO9T was an obligate heterotroph and reduced sulfate. It grew between 35 and 60 °C (optimum 50 °C), at pH 5.4–7.9 (optimum pH 5.9–6.4) and with 1.5–4.5 % NaCl (optimum 2.5 %). The fatty acid composition was C16 : 0 (61.5 %) and 12Me16 : 0 (38.5 %). The DNA G+C content was 34.9 mol%. The 16S rRNA gene sequence analysis indicated that strain TFISO9T belonged to the genus Desulfothermus. Based on physiological and phylogenetic characteristics, strain TFISO9T represents a novel species for which the name Desulfothermus okinawensis sp. nov. is proposed. The type strain is TFISO9T (=JCM 13304T=DSM 17375T).
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