1887

Abstract

Seven bacterial isolates from farmed Atlantic cod displaying chronic granulomatous disease were characterized by phenotypic and molecular taxonomic methods. The isolates were Gram-negative, facultatively intracellular, non-motile, strictly aerobic coccobacilli which produced HS from cysteine-supplemented media and are therefore phenotypically consistent with members of the genus . Comparison of 16S rRNA gene sequences and six partial housekeeping gene sequences (, , , , and ) confirmed the organism as a member of the genus , with as its closest relative (99.3 % 16S rRNA gene sequence similarity, 92.2–99.0 % housekeeping gene sequence similarity). Despite the close relationship with , isolates from Atlantic cod could be readily distinguished phenotypically and genetically from ATCC 25015. DNA–DNA hybridization studies revealed a mean reassociation value of 68 %. Thus, on the basis of phenotypic and molecular genetic evidence, we propose that the strains isolated from Atlantic cod should be recognized as subsp. subsp. nov. with the type strain 2005/50/F292-6C (=NCIMB 14265=LMG 23800). ATCC 25015 (=DSM 735) is reclassified as subsp. subsp. nov.

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2007-09-01
2019-10-20
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vol. , part 9, pp. 1960 - 1965

Primer sequences used in the study. Pairwise similarity (%) between subsp. subsp. nov. 2005/50/F292-6C and members of the genus Kimura two-parameter genetic distances for the strains used in the study. GenBank accession numbers for the sequences determined in this study.

Combined file containing Supplementary Tables S1-S4. [ PDF] (57 KB)



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