1887

Abstract

An orange-pigmented, Gram-positive bacterial strain, designated PgEx11, was isolated from pigeon faeces. Analysis of the 16S rRNA gene sequence of the isolate indicated that it had 94.2–98.2 % sequence identity with respect to those of seven recognized species of the genus . The strain PgEx11 contained anteiso-C as a major cellular fatty acid and MK-7 and MK-8 as the major menaquinones. The DNA G+C content of strain PgEx11 was 50.5 mol%. Furthermore, analysis of the 16S rRNA gene sequence indicated high levels of similarity with (98.2 %), (97.6 %), (97.5 %) and (97.1 %). However, the mean value for DNA–DNA relatedness between PgEx11 and these four closely related species was in the range 45.4–16.8 %, respectively. Moreover, strain PgEx11 also differs from its close relatives with regard to biochemical and chemotaxonomic characteristics. On the basis of phenotypic, chemotaxonomic and genotypic differences, strain PgEx11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PgEx11 (=MTCC 7251=DSM 17517).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64742-0
2007-06-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/6/1266.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64742-0&mimeType=html&fmt=ahah

References

  1. Alam, S. I., Singh, L., Dube, S., Reddy, G. S. N. & Shivaji, S. ( 2003; ). Psychrophilic Planococcus maitriensis sp. nov. from Antarctica. Syst Appl Microbiol 26, 505–510.[CrossRef]
    [Google Scholar]
  2. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  3. Dai, X., Wang, Y.-N., Wang, B.-J., Liu, S.-J. & Zhou, Y.-G. ( 2005; ). Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov. Int J Syst Evol Microbiol 55, 699–702.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Hao, M. V. & Komagata, K. ( 1985; ). A new species of Planococcus, P. kocurii isolated from fish, frozen foods, and fish curing brine. J Gen Appl Microbiol 31, 441–455.[CrossRef]
    [Google Scholar]
  6. Hao, M. V., Kocur, M. & Komagata, K. ( 1984; ). Marinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall; and Marinococcus albus sp. nov. and Marinococcus halophilus Novitsky and Kushner comb. nov. J Gen Appl Microbiol 30, 449–459.[CrossRef]
    [Google Scholar]
  7. Junge, K., Gosink, J. J., Hoppe, H.-G. & Staley, J. T. ( 1998; ). Arthrobacter, Brachybacterium and Planococcus isolates identified from Antarctic sea ice brine. Description of Planococcus mcmeekinii, sp. nov. Syst Appl Microbiol 21, 306–314.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Kocur, M., Pacova, Z., Hodgkiss, W. & Martinec, T. ( 1970; ). The taxonomic status of the genus Planococcus Migula 1894 . Int J Syst Bacteriol 20, 241–248.[CrossRef]
    [Google Scholar]
  10. Komagata, K. & Suzuki, K. I. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–206.
    [Google Scholar]
  11. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  12. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  13. Mayilraj, S., Prasad, G. S., Suresh, K., Saini, H. S., Shivaji, S. & Chakrabarti, T. ( 2005; ). Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55, 91–94.[CrossRef]
    [Google Scholar]
  14. Migula, W. ( 1894; ). Uber ein neues system der bacterien. Arb Bakteriol Inst Karlsruhe 1, 235–238.
    [Google Scholar]
  15. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, K. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  16. Nakagawa, Y., Sakane, T. & Yokota, A. ( 1996; ). Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov. Int J Syst Bacteriol 46, 866–870.[CrossRef]
    [Google Scholar]
  17. Pandey, K. K., Mayilraj, S. & Chakrabarti, T. ( 2002; ). Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52, 1559–1567.[CrossRef]
    [Google Scholar]
  18. Reddy, G. S. N., Prakash, J. S. S., Vairamani, M., Prabhakar, S., Matsumoto, G. I. & Shivaji, S. ( 2002; ). Planococcus antarcticus and Planococcus psychrophilus spp. nov., isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6, 253–261.[CrossRef]
    [Google Scholar]
  19. Romano, I., Giordano, A., Lama, L., Nicolaus, B. & Gambacorta, A. ( 2003; ). Planococcus rifietoensis sp. nov., isolated from algal mat collected from a sulfurous spring in Campania (Italy). Syst Appl Microbiol 26, 357–366.[CrossRef]
    [Google Scholar]
  20. Sato, N. S. & Murata, N. ( 1988; ). Membrane lipids. Methods Enzymol 167, 251–259.
    [Google Scholar]
  21. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  22. Shivaji, S., Ray, M. K., Saisree, L., Jagannadham, M. V., Seshu Kumar, G., Reddy, G. S. N. & Bhargava, P. M. ( 1992; ). Sphingobacterium antarcticus sp. nov. a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42, 102–116.[CrossRef]
    [Google Scholar]
  23. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  24. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  25. Suresh, K., Mayilraj, S. & Chakrabarti, T. ( 2006; ). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family “Flexibacteraceae”. Int J Syst Evol Microbiol 56, 1703–1707.[CrossRef]
    [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  27. Tourova, T. P. & Antonov, A. S. ( 1987; ). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19, 333–355.
    [Google Scholar]
  28. Van de Peer, Y. & De Wachter, R. ( 1997; ). Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13, 227–230.
    [Google Scholar]
  29. Yoon, J.-H., Kang, S.-S., Lee, K.-C., Lee, E. S., Kho, Y. H., Kang, K. H. & Park, Y.-H. ( 2001; ). Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgali, and transfer of Planococcus okeanokoites ( Nakagawa et al. 1996 ) and Planococcus mcmeekinii ( Junge et al. 1998 ) to the genus Planomicrobium. Int J Syst Evol Microbiol 51, 1511–1520.
    [Google Scholar]
  30. Yoon, J.-H., Weiss, N., Kang, K. H., Oh, T. & Park, Y.-H. ( 2003; ). Planococcus maritimus sp. nov., isolated from sea water of a tidal flat in Korea. Int J Syst Evol Microbiol 53, 2013–2017.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64742-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64742-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1266 - 1271

Differential phenotypic and chemotaxonomic characteristics for PgEx11 with respect to species of the genera and [PDF](30 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error