1887

Abstract

Two alkaliphilic, moderately halophilic bacteria, designated BM2 and HN2, were isolated from non-saline forest soil in Japan. The cells of strain BM2 were motile, aerobic, rod-shaped and Gram-positive. The peptidoglycan was of the A1 type, and the diamino acid was -diaminopimelic acid. Growth was observed at NaCl concentrations between 5.0 and 25.0 % (w/v) (the optimum being 10.0–15.0 %, w/v), at pH 7.0–10.0 (optimum, pH 9.0–9.5) and at 20–50 °C. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C and anteiso-C. The G+C content of total DNA of strain BM2 was 37.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain BM2 was most closely related to DSM 5271 (98.0 % sequence similarity). DNA–DNA hybridization results indicated low levels of relatedness between strain BM2 and JCM 12303 (23 and 16 % reciprocally), JCM 13893 (25 and 21 %) and JCM 13894 (27 and 19 %). On the basis of the phylogenetic and phenotypic characteristics, strain BM2 represents a novel species, for which the name sp. nov. is proposed. The type strain is BM2 (=JCM 14193=DSM 18495).

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2007-04-01
2019-10-15
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