1887

Abstract

A Gram-negative bacterium, designated strain EMB117, was isolated from a municipal wastewater treatment plant and characterized by polyphasic taxonomy. The cells were non-spore-forming rods that showed gliding motility. Optimal growth occurred at 25–30 °C and pH 7.0–8.0. Strain EMB117 contained phosphatidylethanolamine as the predominant polar lipid, and the major fatty acids were iso-C, iso-C 3-OH, iso-C 3-OH and summed feature 3 (C 7 and/or iso-C 2-OH). The G+C content of the genomic DNA was 33.5 mol% and the major isoprenoid quinone was MK-6. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB117 belonged to the genus and was most closely related to DSM 425 (97.8 % sequence similarity). The DNA–DNA relatedness between strain EMB117 and ATCC 17061 was about 18 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB117 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is EMB117 (=KCTC 12612=DSM 17963).

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2007-02-01
2019-10-15
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vol. , part 2, pp. 233 - 237

Transmission electron micrograph showing the general morphology of negatively stained cells of strain EMB117 after growth for 1 day at 30 °C in R2A broth. Some cells show a knar. Bar, 1 µm.



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