1887

Abstract

The taxonomic position of , a member of the family found in rabbits, hares and hamsters, has been challenged. 16S rRNA gene () sequence data show the species to be heterogeneous. Using a polyphasic approach, 23 strains that were identified previously as belonging, or closely related, to were analysed. Eighty characters were included in the phenotypic analysis. Phylogenetic analysis was done based on , , and sequences. In addition, the sequence similarities were used to calculate the whole-genome sequence relatedness of all strains investigated as well as that with other members of the family . The phenotypic analysis allowed identification of five groups. The major group of 17 strains could be classified as . Two hamster isolates were closely related to but differed in a few characters. Single isolates from a rabbit and snowshoe-hare were phenotypically related to . One rabbit isolate was related to the genus , while another isolate could not be classified phenotypically with known taxa. The phylogenetic analysis confirmed the phenotypic grouping. In contrast to the -based tree, the strains clustered unambiguously with the type species and related species of the genus in the -, - and -based trees. Genome similarity comparison using finally confirmed the high genomic relationship of the strains with the type species and related species of the genus and allowed a clear assignment of the other unrelated strains to the phenotypic and phylogenetic clusters outlined. The present findings allow the description of to be emended and separate it more clearly from other species, both phenotypically and genotypically. The type strain of is CCUG 12396 (=Frederiksen 243=ATCC 51571=NCTC 11408=CIP 103283).

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2007-03-01
2020-01-22
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Phenotypic characters of the strains investigated. [PDF](28 KB)

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Phylogenetic tree based on the gene. [PDF](17 KB)

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Phylogenetic tree based on the gene. [PDF](17 KB)

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Phylogenetic tree based on the gene. [PDF](17 KB)

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