1887

Abstract

The taxonomic position of , a member of the family found in rabbits, hares and hamsters, has been challenged. 16S rRNA gene () sequence data show the species to be heterogeneous. Using a polyphasic approach, 23 strains that were identified previously as belonging, or closely related, to were analysed. Eighty characters were included in the phenotypic analysis. Phylogenetic analysis was done based on , , and sequences. In addition, the sequence similarities were used to calculate the whole-genome sequence relatedness of all strains investigated as well as that with other members of the family . The phenotypic analysis allowed identification of five groups. The major group of 17 strains could be classified as . Two hamster isolates were closely related to but differed in a few characters. Single isolates from a rabbit and snowshoe-hare were phenotypically related to . One rabbit isolate was related to the genus , while another isolate could not be classified phenotypically with known taxa. The phylogenetic analysis confirmed the phenotypic grouping. In contrast to the -based tree, the strains clustered unambiguously with the type species and related species of the genus in the -, - and -based trees. Genome similarity comparison using finally confirmed the high genomic relationship of the strains with the type species and related species of the genus and allowed a clear assignment of the other unrelated strains to the phenotypic and phylogenetic clusters outlined. The present findings allow the description of to be emended and separate it more clearly from other species, both phenotypically and genotypically. The type strain of is CCUG 12396 (=Frederiksen 243=ATCC 51571=NCTC 11408=CIP 103283).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64659-0
2007-03-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/3/625.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64659-0&mimeType=html&fmt=ahah

References

  1. Acinas S. G., Marcelino L. A., Klepac-Ceraj V., Polz M. F. 2004; Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons. J Bacteriol 186:2629–2635 [CrossRef]
    [Google Scholar]
  2. Arseculeratne S. N. 1962; Actinobacillosis in joints of rabbits. J Comp Pathol 72:33–39 [CrossRef]
    [Google Scholar]
  3. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  4. Bercovier H., Escande F., Grimont P. A. 1984; Biological characterization of Actinobacillus species and Pasteurella ureae . Ann Microbiol 135A:203–218
    [Google Scholar]
  5. Bisgaard M. 1993; Ecology and significance of Pasteurellaceae in animals. Zentralbl Bakteriol 279:7–26 [CrossRef]
    [Google Scholar]
  6. Bisgaard M., Houghton S. B., Mutters R., Stenzel A. 1991; Reclassification of German, British and Dutch isolates of so-called Pasteurella multocida obtained from pneumonic calf lungs. Vet Microbiol 26:115–124 [CrossRef]
    [Google Scholar]
  7. Blackall P. J., Bisgaard M., McKenzie R. A. 1997; Characterisation of Australian isolates of Actinobacillus capsulatus , Actinobacillus equuli , Pasteurella caballi and Bisgaard Taxa 9 and 11. Aust Vet J 75:52–55 [CrossRef]
    [Google Scholar]
  8. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708 [CrossRef]
    [Google Scholar]
  9. Christensen H., Bisgaard M. 2004; Revised definition of Actinobacillus sensu stricto isolated from animals. A review with special emphasis on diagnosis. Vet Microbiol 99:13–30 [CrossRef]
    [Google Scholar]
  10. Christensen H., Bisgaard M., Olsen J. E. 2002; Reclassification of equine isolates previously reported as Actinobacillus equuli , variants of A. equuli , Actinobacillus suis or Bisgaard taxon 11 and proposal of A.equuli subsp. equuli subsp.nov. and A.equuli subsp. haemolyticus subsp. nov.. Int J Syst Evol Microbiol 52:1569–1576 [CrossRef]
    [Google Scholar]
  11. Christie R., Atkins N. E., Munch-Petersen E. 1944; A note on a lytic phenomenon shown by group B streptococci. Aust J Exp Biol 22:197–200 [CrossRef]
    [Google Scholar]
  12. Dewhirst F. E., Paster B. J., Olsen I., Fraser G. J. 1992; Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 174:2002–2013
    [Google Scholar]
  13. Escande F., Grimont F., Grimont P. A. D., Bercovier H. 1984; Deoxyribonucleic acid relatedness among strains of Actinobacillus spp. and Pasteurella ureae . Int J Syst Bacteriol 34:309–315 [CrossRef]
    [Google Scholar]
  14. Korczak B., Christensen H., Emler S., Frey J., Kuhnert P. 2004; Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol 54:1393–1399 [CrossRef]
    [Google Scholar]
  15. Korczak B. M., Stieber R., Emler S., Burnens A. P., Frey J., Kuhnert P. 2006; Genetic relatedness within the genus Campylobacter inferred from rpoB sequences. Int J Syst Evol Microbiol 56:937–945 [CrossRef]
    [Google Scholar]
  16. Krause T., Kunstyr I., Mutters R. 1989; Characterization of some previously unclassified Pasteurellaceae isolated from hamsters. J Appl Bacteriol 67:171–175 [CrossRef]
    [Google Scholar]
  17. Kuhnert P., Korczak B. M. 2006; Prediction of whole-genome DNA–DNA similarity, determination of G+C content and phylogenetic analysis within the family Pasteurellaceae by multilocus sequence analysis (MLSA. Microbiology 152:2537–2548 [CrossRef]
    [Google Scholar]
  18. Kuhnert P., Frey J., Lang N. P., Mayfield L. 2002; Phylogenetic analysis of Prevotella nigrescens , Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering. Int J Syst Evol Microbiol 52:1391–1395 [CrossRef]
    [Google Scholar]
  19. Kuhnert P., Korczak B., Falsen E., Straub R., Hoops A., Boerlin P., Frey J., Mutters R. 2004; Nicoletella semolina gen. nov., sp. nov. a new member of Pasteurellaceae isolated from horses with airway disease. J Clin Microbiol 42:5542–5548 [CrossRef]
    [Google Scholar]
  20. Mutters R., Mannheim W., Bisgaard M. 1989; Taxonomy of the group. In Pasteurella and Pasteurellosis pp  3–34 Edited by Adlam C., Rutter J. M. London: Academic Press;
    [Google Scholar]
  21. Nørskov-Lauritsen N., Christensen H., Okkels H., Kilian M., Bruun B. 2004; Delineation of the genus Actinobacillus by comparison of partial infB sequences. Int J Syst Evol Microbiol 54:635–644 [CrossRef]
    [Google Scholar]
  22. Olsen I., Moller K. 2005 Genus II Actinobacillus Brumpt 1910, 849AL. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 2, part B pp  866–883 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  23. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  24. Yap W. H., Zhang Z., Wang Y. 1999; Distinct types of rRNA operons exist in the genome of the actinomycete Thermomonospora chromogena and evidence for horizontal transfer of an entire rRNA operon. J Bacteriol 181:5201–5209
    [Google Scholar]
  25. Zarnke R. L., Schlater L. 1988; Actinobacillosis in free-ranging snowshoe hares ( Lepus americanus ) from Alaska. J Wildl Dis 24:176–177 [CrossRef]
    [Google Scholar]
  26. Zeigler D. R. 2003; Gene sequences useful for predicting relatedness of whole genomes in bacteria. Int J Syst Evol Microbiol 53:1893–1900 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64659-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64659-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Supplementary material 4

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error