1887

Abstract

A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterial strain, designated RL80JIV, was isolated from a geothermally active underground mine in Japan. Cells were rod-shaped and motile. The temperature and pH ranges for growth were 61–80 °C (optimum at 69–72 °C) and pH 6.4–7.9 (optimum at pH 6.8–7.3), and the strain tolerated up to 0.5 % NaCl. Strain RL80JIV utilized sulfate, sulfite, thiosulfate and elemental sulfur as electron acceptors. Electron donors utilized were H in the presence of CO, and carboxylic acids. Fermentative growth occurred on lactate and pyruvate. The cell wall contained -diaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone MK-7. Major whole-cell fatty acids were iso-C, iso-C and C. Strain RL80JIV was found to be affiliated with the thiosulfate-reducer DSM 14490 (90.9 % 16S rRNA gene sequence similarity) and with the sulfate-reducer DSM 10259 (90.0 % similarity). Strain RL80JIV is therefore considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is RL80JIV (=DSM 16036=JCM 13928).

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2007-01-01
2019-10-16
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