1887

Abstract

An extremely haloalkaliphilic archaeon, strain AD2, was isolated from Aiding salt lake in Xin-Jiang, China. Strain AD2 required at least 12 % NaCl for growth. MgCl was not required. The isolate was able to grow over a pH range of 8.0–10.0 and temperature range of 20–55 °C, with optimal growth at pH 8.7–9.2 and 44–47 °C. The major polar lipids of strain AD2 were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; glycolipids were not detected. Analysis of its 16S rRNA gene sequence indicated that strain AD2 was phylogenetically related to members of the genus , with sequence similarities to the type strains of , and of 97.1, 95.9 and 96.1 %, respectively. The G+C content of its DNA was 60.9 mol% ( ). Levels of DNA–DNA relatedness between strain AD2 and the type strains of , and were 49, 38 and 41 %, respectively. It was concluded that strain AD2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AD2 (=CGMCC 1.6307=JCM 14089).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64651-0
2007-04-01
2021-03-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/4/738.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64651-0&mimeType=html&fmt=ahah

References

  1. Cui H.-L., Tohty D., Feng J., Zhou P.-J., Liu S.-J. 2006a; Natronorubrum aibiense sp. nov., an extremely halophilic archaeon isolated from Aibi salt lake in Xin-Jiang, China, and emended description of the genus Natronorubrum . Int J Syst Evol Microbiol 56:1515–1517 [CrossRef]
    [Google Scholar]
  2. Cui H.-L., Yang Y., Tohty D., Zhou P.-J., Liu S.-J. 2006b; Biodiversity of halophilic archaea isolated from two salt lakes in Xin-Jiang region of China. Wei Sheng Wu Xue Bao 46:171–176
    [Google Scholar]
  3. Fan H.-P., Xue Y.-F., Zeng Y., Zhou P.-J., Ma Y.-H. 2003; Archaeal diversity of Zabuye lake in Tibet analyzed by culture-independent approach. Wei Sheng Wu Xue Bao 43:401–408
    [Google Scholar]
  4. Feng J., Zhou P.-J., Liu S.-J., Warren-Rhodes K. 2005; Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55:149–152 [CrossRef]
    [Google Scholar]
  5. Kates M. 1986 Techniques of Lipidology , 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  6. Kocur M., Hodgkiss W. 1973; Taxonomic status of the genus Halococcus Schoop. Int J Syst Bacteriol 23:151–156 [CrossRef]
    [Google Scholar]
  7. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  8. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  9. Oren A., Ventosa A., Grant W. D. 1997; Proposal of minimal standards for the description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  10. Pan H.-L., Zhou C., Wang H.-L., Xue Y. F., Ma Y.-H. 2006; Diversity of halophilic archaea in hypersaline lakes of Inner Mongolia, China. Wei Sheng Wu Xue Bao 46:1–6
    [Google Scholar]
  11. Tohty D., Xu X.-J. 2001; The numerical distribution of halophilic bacteria and halotolerant bacteria in Aydin Lake and the surrounding area. Acta Ecol Sin 21:1388–1391
    [Google Scholar]
  12. Xu Y., Zhou P.-J., Tian X.-Y. 1999; Characterization of two novel haloalkaliphilic archaea Natronorubrum bangense gen.nov., sp. nov. and Natronorubrum tibetense gen. nov., sp. nov. Int J Syst Bacteriol 49:261–266 [CrossRef]
    [Google Scholar]
  13. Xu Y., Wang Z.-X., Xue Y.-F., Zhou P.-J., Ma Y.-H., Ventosa A., Grant W. D. 2001; Natrialba hulunbeirensis sp. nov. and Natrialba chahannaoensis sp. nov., novel haloalkaliphilic archaea from soda lakes in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 51:1693–1698 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64651-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64651-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error