Bacterial strains Eant 3-9 and Eant 3-3, isolated from the gut of the termite , were characterized using a polyphasic taxonomic approach. Cells of these organisms were Gram-negative, non-pigmented, rod-shaped, non-endospore-forming and facultatively anaerobic. Phenotypic, physiological and genetic characteristics of the two strains were highly similar. Phylogenetic analyses using 16S rRNA gene sequences showed that strain Eant 3-9 formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus ; its closest neighbour was DSM 16940 (98.1 % similarity). Genomic DNA–DNA relatedness between strains Eant 3-9 and Eant 3-3 was 96±4 %, indicating that they belong to a single species. Eant 3-9 displayed DNA–DNA relatedness values of 48±5 and 14±3 % with DSM 16940 and ATCC 13311, respectively, indicating that Eant 3-9 warrants novel species status in the genus . Eant 3-9 and Eant 3-3 could be further distinguished from DSM 16940 using whole-cell protein profiles and a range of physiological and biochemical characteristics. It is evident from genotypic and phenotypic data that Eant 3-9 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is Eant 3-9 (=BCRC 17577=LMG 23580); Eant 3-3 is a reference strain.


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Electrophoretic protein patterns of ATCC 13311 (lane 1), DSM 16940 (lane 2), Eant 3-3 (lane 3) and Eant 3-9 (lane 4). Samples were electrophoresed on a 10 % polyacryamide gel that was stained with Coomassie brilliant blue. Lane M, molecular mass marker (sizes in kDa).


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