1887

Abstract

Bacterial strains Eant 3-9 and Eant 3-3, isolated from the gut of the termite , were characterized using a polyphasic taxonomic approach. Cells of these organisms were Gram-negative, non-pigmented, rod-shaped, non-endospore-forming and facultatively anaerobic. Phenotypic, physiological and genetic characteristics of the two strains were highly similar. Phylogenetic analyses using 16S rRNA gene sequences showed that strain Eant 3-9 formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus ; its closest neighbour was DSM 16940 (98.1 % similarity). Genomic DNA–DNA relatedness between strains Eant 3-9 and Eant 3-3 was 96±4 %, indicating that they belong to a single species. Eant 3-9 displayed DNA–DNA relatedness values of 48±5 and 14±3 % with DSM 16940 and ATCC 13311, respectively, indicating that Eant 3-9 warrants novel species status in the genus . Eant 3-9 and Eant 3-3 could be further distinguished from DSM 16940 using whole-cell protein profiles and a range of physiological and biochemical characteristics. It is evident from genotypic and phenotypic data that Eant 3-9 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is Eant 3-9 (=BCRC 17577=LMG 23580); Eant 3-3 is a reference strain.

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2007-04-01
2021-10-27
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References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
    [Google Scholar]
  2. Chen W. M., Laevens S., Lee T. M., Coenye T., de Vos P., Mergeay M., Vandamme P. 2001; Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51:1729–1735 [CrossRef]
    [Google Scholar]
  3. Chung Y. C., Kobayashi T., Kanai H., Akiba T., Kudo T. 1995; Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococccus profundus DT5432. Appl Environ Microbiol 61:1502–1506
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Farmer J. J. III, Davis B. R., Hickman-Brenner F. W., McWhorter A., Huntley-Carter G. P., Asbury M. A., Riddle C., Wathen-Grady H. G., Elias C. other authors 1985; Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol 21:46–76
    [Google Scholar]
  6. Fernandez M. P., Meugnier H., Grimont P. A. D., Bardin R. 1989; Deoxyribonucleic acid relatedness among members of the genus Frankia . Int J Syst Bacteriol 39:424–429 [CrossRef]
    [Google Scholar]
  7. Hall T. A. 1999; bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol  3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Lindquist J. A., Farmer J. J. III 2005; Genus XXXVIII. Trabulsiella . In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol. 2, part B pp  827–828 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
  10. McWhorter A. C., Haddock R. L., Nocon F. A., Steigerwalt A. G., Brenner D. J., Aleksic S., Bockemühl J., Farmer J. J III. 1991; Trabulsiella guamensis , a new genus and species of the family Enterobacteriaceae that resembles Salmonella subgroups 4 and 5. J Clin Microbiol 29:1480–1485
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Pot B., Vandamme P., Kersters K. 1994 Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods ( Chemical Methods in Prokaryotic Systematics Series) pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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