1887

Abstract

A Gram-positive, endospore-forming, alkalitolerant bacterial strain, designated MLB2, was isolated from soil from Leh, India, and was subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus . Growth was observed at pH 7.0–11.0, but not at pH 6.0. The DNA G+C content was 41.4 mol%. The highest level of 16S rRNA gene sequence similarity was with JCM 12663 (98.8 %). However, DNA–DNA hybridization experiments indicated low levels of genomic relatedness with the type strains of (62 %), (55 %), (51 %) and (34 %), the species with which strain MLB2 formed a coherent cluster (based on the results of the phylogenetic analysis). On the basis of the phenotypic characteristics and genotypic distinctiveness of strain MLB2, it should be classified within a novel species of , for which the name sp. nov. is proposed. The type strain is MLB2 (=MTCC 7633=JCM 13820).

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2007-02-01
2021-03-07
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