1887

Abstract

A Gram-negative, chemoheterotrophic, facultatively anaerobic coccus, designated IMCC1545, was isolated from the digestive tract of a marine clamworm, , inhabiting a tidal flat of the Yellow Sea. Cells of strain IMCC1545 are non-motile, dividing by binary fission. The predominant fatty acids are anteiso-C and C. The respiratory quinone is menaquinone-7 and the DNA G+C content is 52.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences using three treeing algorithms revealed that the strain formed a novel genus-level lineage within the phylum ‘’. The most closely related named organisms to strain IMCC1545 are ‘’ SI-1234 (86.5 % 16S rRNA gene sequence similarity), ADT3 (81.8 %) and PB90-1 (80.3 %), which belong to subdivision 4 of the ‘’. Subdivision 4 of the ‘’ (here named classis nov.) was divided into two clades, a clade containing strain IMCC1545 and a clade containing . From the taxonomic data obtained in this study, it is proposed that the new marine isolate be placed into a novel genus and species named gen. nov., sp. nov. (the type strain of is IMCC1545=KCCM 42343=NBRC 101964) within fam. nov and ord. nov in the class . The family fam. nov. and order ord. nov. are also formally proposed.

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2007-03-01
2020-01-26
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References

  1. Chin, K. J., Hahn, D., Hengstmann, U., Liesack, W. & Janssen, P. H. ( 1999; ). Characterization and identification of numerically abundant culturable bacteria from the anoxic bulk soil of rice paddy microcosms. Appl Environ Microbiol 65, 5042–5049.
    [Google Scholar]
  2. Chin, K. J., Liesack, W. & Janssen, P. H. ( 2001; ). Opitutus terrae gen. nov., sp. nov., to accommodate novel strains of the division ‘Verrucomicrobia’ isolated from rice paddy soil. Int J Syst Evol Microbiol 51, 1965–1968.[CrossRef]
    [Google Scholar]
  3. Cho, J. C. & Giovannoni, S. J. ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef]
    [Google Scholar]
  4. Cho, J. C., Vergin, K. L., Morris, R. M. & Giovannoni, S. J. ( 2004; ). Lentisphaera araneosa gen. nov., sp. nov., a transparent exopolymer producing marine bacterium, and the description of a novel bacterial phylum, Lentisphaerae. Environ Microbiol 6, 611–621.[CrossRef]
    [Google Scholar]
  5. Derrien, M., Vaughan, E. E., Plugge, C. M. & de Vos, W. M. ( 2004; ). Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium. Int J Syst Evol Microbiol 54, 1469–1476.[CrossRef]
    [Google Scholar]
  6. Freitag, T. E. & Prosser, J. I. ( 2003; ). Community structure of ammonia-oxidizing bacteria within anoxic marine sediments. Appl Environ Microbiol 69, 1359–1371.[CrossRef]
    [Google Scholar]
  7. Garrity, G. M., Bell, J. A. & Lilburn, T. G. ( 2003; ). Taxonomic outline of the procaryotes, release 4.0. New York: Springer.
  8. Haukka, K., Heikkinen, E., Kairesalo, T., Karjalainen, H. & Sivonen, K. ( 2005; ). Effect of humic material on the bacterioplankton community composition in boreal lakes and mesocosms. Environ Microbiol 7, 620–630.[CrossRef]
    [Google Scholar]
  9. Hedlund, B. P., Gosink, J. J. & Staley, J. T. ( 1996; ). Phylogeny of Prosthecobacter, the fusiform caulobacters: members of a recently discovered division of the bacteria. Int J Syst Bacteriol 46, 960–966.[CrossRef]
    [Google Scholar]
  10. Hedlund, B. P., Gosink, J. J. & Staley, J. T. ( 1997; ). Verrucomicrobia div. nov., a new division of the bacteria containing three new species of Prosthecobacter. Antonie van Leeuwenhoek 72, 29–38.[CrossRef]
    [Google Scholar]
  11. Hugenholtz, P., Goebel, B. M. & Pace, N. R. ( 1998; ). Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol 180, 4765–4774.
    [Google Scholar]
  12. Janssen, P. H., Schuhmann, A., Morschel, E. & Rainey, F. A. ( 1997; ). Novel anaerobic ultramicrobacteria belonging to the Verrucomicrobiales lineage of bacterial descent isolated by dilution culture from anoxic rice paddy soil. Appl Environ Microbiol 63, 1382–1388.
    [Google Scholar]
  13. Ludwig. W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. O'Farrell, K. A. & Janssen, P. H. ( 1999; ). Detection of Verrucomicrobia in a pasture soil by PCR-mediated amplification of 16S rRNA genes. Appl Environ Microbiol 65, 4280–4284.
    [Google Scholar]
  16. Sakai, T., Ishizuka, K. & Kato, I. ( 2003; ). Isolation and characterization of a fucoidan-degrading marine bacterium. Mar Biotechnol 5, 409–416.[CrossRef]
    [Google Scholar]
  17. Sangwan, P., Chen, X., Hugenholtz, P. & Janssen, P. H. ( 2004; ). Chthoniobacter flavus gen. nov., sp. nov., the first pure-culture representative of subdivision two, Spartobacteria classis nov., of the phylum Verrucomicrobia. Appl Environ Microbiol 70, 5875–5881.[CrossRef]
    [Google Scholar]
  18. Schlesner, H. ( 1987; ). Verrucomicrobium spinosum gen. nov., sp. nov.; a fimbriated prosthecate bacterium. Syst Appl Microbiol 10, 54–56.[CrossRef]
    [Google Scholar]
  19. Shieh, W. Y. & Jean, W. D. ( 1998; ). Alterococcus agarolyticus, gen. nov., sp. nov., a halophilic thermophilic bacterium capable of agar degradation. Can J Microbiol 44, 637–645.[CrossRef]
    [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic Characterization. Washington, DC: American Society for Microbiology.
  21. Swofford, D. ( 2002; ). paup* Phylogenetic Analysis Using Parsimony (and other methods). Sunderland, MA: Sinauer Associates.
  22. Ward-Rainey, N., Rainey, F. A., Schlesner, H. & Stackebrandt, E. ( 1995; ). Assignment of hitherto unidentified 16S rDNA species to a main line of descent within the domain Bacteria. Microbiology 141, 3247–3250.[CrossRef]
    [Google Scholar]
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