1887

Abstract

Strain PRAA4-1, a motile, Gram-negative, violet-pigmented bacterium, was isolated from Maryland forest soil and found to be orally toxic to Colorado potato beetle larvae and other insects. Morphological, biological, biochemical and molecular characterization revealed that this strain was most similar to , the type species and only currently recognized member of the genus . DNA–DNA hybridization with ATCC 12472 was 27 %. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain PRAA4-1 and form a monophyletic clade, with the closest ancestral taxon within the . On the basis of phenotypic, genotypic and phylogenetic analyses, strain PRAA4-1 (=NRRL B-30655=DSM 17043) is proposed as the type strain of a novel species of the genus , sp. nov.

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2007-05-01
2019-09-21
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References

  1. Atlas, R. M. ( 2004; ). Handbook of Microbiological Media, 3rd edn. Boca Raton, FL: CRC Press.
  2. Bell, R. A., Owens C. D., Shapiro, M. & Tardif, J. G. R. ( 1981; ). Development of mass rearing technology. In The Gypsy Moth: Research Toward Integrated Pest Management, Technical Bulletin no. 1584, pp. 599–633. Edited by C. C. Doane & M. L. McManus. Washington, DC: US Department of Agriculture.
  3. Brazilian National Genome Project Consortium ( 2003; ). The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability. Proc Natl Acad Sci U S A 100, 11660–11665.[CrossRef]
    [Google Scholar]
  4. Bucher, G. E. ( 1981; ). Identification of bacteria found in insects. In Microbial Control of Pests and Plant Diseases 1970–1980, pp. 7–33. Edited by H. D. Burges. New York: Academic Press.
  5. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  6. Durán, N. & Menck, C. F. ( 2001; ). Chromobacterium violaceum: a review of pharmacological and industrial perspectives. Crit Rev Microbiol 27, 201–222.[CrossRef]
    [Google Scholar]
  7. Durán, N., Antonio, R. V., Haun, M. & Pilli, R. A. ( 1994; ). Biosynthesis of a trypanocide by Chromobacterium violaceum. World J Microbiol Biotechnol 10, 686–690.[CrossRef]
    [Google Scholar]
  8. Dutky, S. R. ( 1940; ). Two new spore-forming bacteria causing milky diseases of Japanese beetle larvae. J Agric Res 61, 57–68.
    [Google Scholar]
  9. Farrar, R. R., Jr, Martin, P. A. W. & Ridgway, R. L. ( 2001; ). A strain of Serratia marcescens (Enterobacteriaceae) with high virulence per os to larvae of a laboratory colony of the corn earworm (Lepidoptera: Noctuidae). J Entomol Sci 36, 380–390.
    [Google Scholar]
  10. Forbes, L. ( 1981; ). Rapid flagella stain. J Clin Microbiol 13, 807–809.
    [Google Scholar]
  11. Forst, S. & Nealson, K. ( 1996; ). Molecular biology of the symbiotic-pathogenic bacteria Xenorhabdus spp. and Photorhabdus spp. Microbiol Rev 60, 21–43.
    [Google Scholar]
  12. Gelman, D. B., Bell, R. A., Liska, L. J. & Hu, J. S. ( 2001; ). Artificial diets for rearing Colorado potato beetle, Leptinotarsa decemlineata. J Insect Sci 1, 7.
    [Google Scholar]
  13. Gillis, M. & Logan, N. A. ( 2005; ). Genus IV. Chromobacterium Bergonzini 1881, 153AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 824–827. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  14. Grimes, D. J., Woese, C. R., MacDonell, M. T. & Colwell, R. R. ( 1997; ). Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov. Int J Syst Bacteriol 47, 19–27.[CrossRef]
    [Google Scholar]
  15. Grimont, P. A. D. & Grimont, F. ( 1978; ). The genus Serratia. Annu Rev Microbiol 32, 221–248.[CrossRef]
    [Google Scholar]
  16. Hoshino, T., Takano, T., Hori, S. & Ogasawara, N. ( 1987; ). Biosynthesis of violacein: origins of the hydrogen, nitrogen and oxygen atoms in the 2-pyrrolidone nucleus. Agric Biol Chem 51, 2733–2741.[CrossRef]
    [Google Scholar]
  17. Hungria, M., Astolfi-Filho, S., Chueire, L. M. O., Nicolás, M. F., Santos, E. B. P., Bulbol, M. R., Souza-Filho, A., Nogueira Assunção, E., Germno, M. G. & Vasconcelos, A. T. R. ( 2005; ). Genetic characterization of Chromobacterium isolates from black water environments in the Brazilian Amazon. Lett Appl Microbiol 41, 17–23.[CrossRef]
    [Google Scholar]
  18. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  19. Jackson, T. A., Huger, A. M. & Glare, T. R. ( 1993; ). Pathology of amber disease in the New Zealand grass grub Costelytra zealandica (Coleoptera: Scarabaidae). J Invertebr Pathol 61, 123–130.[CrossRef]
    [Google Scholar]
  20. Lee, I.-M., Hammond, R. W., Davis, R. E. & Gundersen, D. E. ( 1993; ). Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. Phytopathology 83, 834–842.[CrossRef]
    [Google Scholar]
  21. Martin, P. A. W. ( 2004a; ). A freeze-dried diet to test pathogens of Colorado potato beetle. Biol Control 29, 109–114.[CrossRef]
    [Google Scholar]
  22. Martin, P. A. W. ( 2004b; ). A stilbene optical brightener can enhance bacterial pathogenicity to gypsy moth (Lepidoptera: Lymantriidae) and Colorado potato beetle (Coleoptera: Chrysomelidae). Biocontrol Sci Technol 14, 375–383.[CrossRef]
    [Google Scholar]
  23. Martin, P. A. W., Blackburn, M. & Shropshire, A. D. ( 2004; ). Two new bacterial pathogens of Colorado potato beetle (Coleoptera: Chrysomelidae). J Econ Entomol 97, 774–780.[CrossRef]
    [Google Scholar]
  24. McClean, K. H., Winson, M. K., Fish, L., Taylor, A., Chhabra, S. R., Camara, M., Daykin, M., Lamb, J. H., Swift, S. & other authors ( 1997; ). Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143, 3703–3711.[CrossRef]
    [Google Scholar]
  25. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  26. MIDI ( 2002; ). Sherlock Microbial Identification System version 4.5. MIS Operating Manual. Newark, DE: MIDI, Inc.
  27. Moore, D. D. ( 1995; ). Preparation and analysis of DNA. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.2. Edited by F. W. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Wiley.
  28. Nei, M. & Li, W.-H. ( 1979; ). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A 76, 5269–5273.[CrossRef]
    [Google Scholar]
  29. SAS Institute ( 2004; ). SAS OnlineDoc®. Version 9.1. Cary, SC: SAS Institute Inc.
  30. Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R. & Dean, D. H. ( 1998; ). Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62, 775–806.
    [Google Scholar]
  31. Scholz, H. C., Witte, A., Tomaso, H., Al Dahouk, S. & Neubauer, H. ( 2005; ). Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis. FEMS Microbiol Lett 244, 347–352.[CrossRef]
    [Google Scholar]
  32. Schroder, R. F. W., Martin, P. A. W. & Athanas, M. M. ( 2001; ). Effect of a phloxine B–cucurbitacin bait on diabroticite beetles (Coleoptera: Chrysomelidae). J Econ Entomol 94, 892–897.[CrossRef]
    [Google Scholar]
  33. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  34. Swofford, D. L. ( 2002; ). paup*: Phylogenetic Analysis Using Parsimony (and other methods) 4.0 beta for Macintosh. Sunderland, MA: Sinauer Associates.
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  36. Wergin, W. P. & Erbe, E. F. ( 1991; ). Increasing resolution and versatility in low temperature conventional and field emission scanning electron microscopy. Scanning Microsc 5, 927–936.
    [Google Scholar]
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Electron micrograph of cells of strain PRAA4-1 grown for 5 days on L agar. Bar, 1.5 µm. Magnification, ×20000.

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Colonies of strain PRAA4-1 on L agar after 24 h. Violet centres surrounded by creamy growth (b) compared with violet colonies of ATCC 12472 (a) grown under the same conditions.

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Colonies of strain PRAA4-1 on L agar after 24 h. Violet centres surrounded by creamy growth (b) compared with violet colonies of ATCC 12472 (a) grown under the same conditions.

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Southern hybridization analysis of genomic DNA isolated from strain PRAA4-1 and ATCC 12472 . [PDF](27 KB)

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Cellular fatty acid composition of strain PRAA4-1 compared with that of ATCC 12472 and related taxa. [ PDF] (20 KB)

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