1887

Abstract

An obligate anaerobic, mesophilic, motile and endospore-forming bacterium, designated 1D, was isolated from a subsurface soil sample. The young culture of strain 1D was Gram-positive and formed oval spores that were central in position. Based on the biochemical, chemotaxonomic and physiological data, strain 1D appears to be a member of the genus . Strain 1D was found to be capable of degrading -nitrophenol (pNP) at a concentration of 0.5 mM under anaerobic conditions as revealed by HPLC analysis. The major fatty acids were C(28.02 %), iso-C I/anteiso B (23.05 %) and C (10.02 %). The major polar lipid content was diphosphatidylglycerol. Strain 1D showed highest 16S rRNA gene sequence similarity to JW/YJL-B3 (98.2 %) and similarity was less for ATCC 25775 (95.1 %), SL1 (95.0 %) and P7 (95.0 %). Phylogenetic analysis showed that it formed a coherent cluster with the species belonging to cluster I of the genus . The DNA G+C content was 35.5 mol%. DNA–DNA hybridization analysis indicated a mean value of 36.4 % between strain 1D and its closest relative . Several phenotypic differences from the closely related species were also revealed. On the basis of the polyphasic characteristics, strain 1D represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1D (=MTCC 7832=JCM 14030).

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2007-08-01
2019-10-18
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vol. , part 8, pp. 1886 - 1890

Cellular fatty acid (%) composition of strain 1D and [PDF](64 KB)



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