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Abstract

A novel, moderately halophilic, Gram-positive coccus, designated strain S2R53-5, was isolated from jeotgal, a traditional Korean fermented seafood. The organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. Strain S2R53-5 grew in the presence of 0.5–15 % (w/v) NaCl and at pH 6.5–11.0, with optimum growth at 5 % (w/v) NaCl and pH 7.0. The temperature range for growth was 20.0–30.0 °C, with an optimum temperature of 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S2R53-5 belongs to the family and was most closely related to DSM 5351 (96.8 % gene sequence similarity), DSM 5352 (96.1 %), T8 (95.2 %) and YKJ-101 (95.1 %). The genomic DNA G+C content was 47.0 mol%, which is in the range of 46–51 mol% that is characteristic for the genus . Levels of DNA–DNA relatedness between strain S2R53-5 and DSM 5351, DSM 5352 and KCTC 13928 were 32.2, 15.4 and 4.6 %, respectively. Chemotaxonomic data (major menaquinone, MK-6; major fatty acids, iso-C and anteiso-C; cell-wall murein type, Lys and Gly) and 16S rRNA gene sequence analysis supported the affiliation of strain S2R53-5 with the genus . The combined evidence from the low DNA–DNA relatedness, physiological, biochemical and other genotypic data indicate that strain S2R53-5 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S2R53-5 (=KCTC 13030=LMG 23640).

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2007-03-01
2019-10-14
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vol. , part 3, pp. 633 - 638

Comparison of selected characteristics of S2R53-5 with members of the genus and other related genera.

Cellular fatty acid profiles of S2R53-5 and related taxa.

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