1887

Abstract

A novel psychrophilic, yellow-pigmented and obligate aerobic bacterium, strain 0499, was isolated from the China No.1 glacier. Strain 0499 displayed the common phenotypic and chemotaxonomic features of the genus , containing menaquinone-6 (MK-6) as the major quinone and C, iso-C, C 6 and summed feature 3 (C 7/iso-C 2-OH) as the major fatty acids. Optimal growth occurred at 21 °C. The genomic DNA G+C content was 36.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0499 was related to members of the genus , sharing the highest sequence similarities with (97.9 %), (97.4 %) and (97.2 %). On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, a novel species is proposed with strain 0499 (=CGMCC 1.5380=JCM 13953) as the type strain.

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2006-12-01
2024-11-05
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References

  1. Aslam Z., Im W.-T., Kim M. K., Lee S.-T. 2005; Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55:747–751 [CrossRef]
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. (editors) 1923 Genus II. Flavobacterium gen.nov. In Bergey's Manual of Determinative Bacteriology pp  1–442 Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Bernardet J. F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  6. Dong X.-Z., Cai M.-Y. (editors) 2001; Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria pp  370–398 Beijing: Science Press (in Chinese;
    [Google Scholar]
  7. Horn M. A., Ihssen J., Matthies C., Schramm A., Acker G., Drake H. L. 2005; Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa . Int J Syst Evol Microbiol 55:1255–1265 [CrossRef]
    [Google Scholar]
  8. Huß V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  11. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  12. Liu H., Xu Y., Ma Y., Zhou P. 2000; Characterization of Micrococcus antarcticus sp. nov., a psychrophilic bacterium from Antarctica. Int J Syst Evol Microbiol 50:715–719 [CrossRef]
    [Google Scholar]
  13. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 501055–1063 [CrossRef]
    [Google Scholar]
  14. Nogi Y., Soda K., Oikawa T. 2005; Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst Appl Microbiol 28:310–315 [CrossRef]
    [Google Scholar]
  15. Reichenbach H. 1989; Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology vol  3 pp  2011–2013 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning : a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  19. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. 1986; The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5:139–156 [CrossRef]
    [Google Scholar]
  20. Van Trappen S., Mergaert J., Swings J. 2003; Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53:1241–1245 [CrossRef]
    [Google Scholar]
  21. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2005; Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55:769–772 [CrossRef]
    [Google Scholar]
  22. Wang Z. W., Liu H. Y., Dai X., Wang B. J., Jiang C. Y., Liu S.-J. 2006; Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56:439–442 [CrossRef]
    [Google Scholar]
  23. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989 Analysis of menaquinone compound in microbial cells by HPLC. Microbiology (English translation of Mikrobiologiya ) 16176–178
  24. Yi H., Oh H.-M., Lee J.-H., Kim S.-J., Chun J. 2005; Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55:637–641 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kang S.-J., Oh T.-K. 2006; Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:997–1000 [CrossRef]
    [Google Scholar]
  26. Zhu F., Wang S., Zhou P. 2003; Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53:853–857 [CrossRef]
    [Google Scholar]
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