1887

Abstract

A novel psychrophilic, yellow-pigmented and obligate aerobic bacterium, strain 0499, was isolated from the China No.1 glacier. Strain 0499 displayed the common phenotypic and chemotaxonomic features of the genus , containing menaquinone-6 (MK-6) as the major quinone and C, iso-C, C 6 and summed feature 3 (C 7/iso-C 2-OH) as the major fatty acids. Optimal growth occurred at 21 °C. The genomic DNA G+C content was 36.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0499 was related to members of the genus , sharing the highest sequence similarities with (97.9 %), (97.4 %) and (97.2 %). On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, a novel species is proposed with strain 0499 (=CGMCC 1.5380=JCM 13953) as the type strain.

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2006-12-01
2019-10-20
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References

  1. Aslam, Z., Im, W.-T., Kim, M. K. & Lee, S.-T. ( 2005; ). Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55, 747–751.[CrossRef]
    [Google Scholar]
  2. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M. (editors) ( 1923; ). Genus II. Flavobacterium gen. nov. In Bergey's Manual of Determinative Bacteriology, pp. 1–442. Baltimore: Williams & Wilkins.
  3. Bernardet, J. F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  4. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Dong, X.-Z. & Cai, M.-Y. (editors) ( 2001; ). Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing: Science Press (in Chinese).
  7. Horn, M. A., Ihssen, J., Matthies, C., Schramm, A., Acker, G. & Drake, H. L. ( 2005; ). Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa. Int J Syst Evol Microbiol 55, 1255–1265.[CrossRef]
    [Google Scholar]
  8. Huß, V. A. R., Festl, H. & Schleifer, K.-H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  12. Liu, H., Xu, Y., Ma, Y. & Zhou, P. ( 2000; ). Characterization of Micrococcus antarcticus sp. nov., a psychrophilic bacterium from Antarctica. Int J Syst Evol Microbiol 50, 715–719.[CrossRef]
    [Google Scholar]
  13. McCammon, S. A. & Bowman, J. P. ( 2000; ). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063.[CrossRef]
    [Google Scholar]
  14. Nogi, Y., Soda, K. & Oikawa, T. ( 2005; ). Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst Appl Microbiol 28, 310–315.[CrossRef]
    [Google Scholar]
  15. Reichenbach, H. ( 1989; ). Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2013. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  18. Sasser, M. ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  19. Sly, L. I., Blackall, L. L., Kraat, P. C., Tian-Shen, T. & Sangkhobol, V. ( 1986; ). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5, 139–156.[CrossRef]
    [Google Scholar]
  20. Van Trappen, S., Mergaert, J. & Swings, J. ( 2003; ). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53, 1241–1245.[CrossRef]
    [Google Scholar]
  21. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2005; ). Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55, 769–772.[CrossRef]
    [Google Scholar]
  22. Wang, Z. W., Liu, H. Y., Dai, X., Wang, B. J., Jiang, C. Y. & Liu, S.-J. ( 2006; ). Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56, 439–442.[CrossRef]
    [Google Scholar]
  23. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology (English translation of Mikrobiologiya) 16, 176–178.
    [Google Scholar]
  24. Yi, H., Oh, H.-M., Lee, J.-H., Kim, S.-J. & Chun, J. ( 2005; ). Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55, 637–641.[CrossRef]
    [Google Scholar]
  25. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2006; ). Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 997–1000.[CrossRef]
    [Google Scholar]
  26. Zhu, F., Wang, S. & Zhou, P. ( 2003; ). Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53, 853–857.[CrossRef]
    [Google Scholar]
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vol. , part 12, pp. 2921 - 2925

Comparison of cellular fatty acid content (%) of sp. nov., its close phylogenetic relatives and some other psychrophilic species. [PDF](84 KB)



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