1887

Abstract

Strain CSC1, a Gram-negative, aerobic, methane-oxidizing bacterium, was isolated from an uncontaminated aquifer nearly 20 years ago. Based on 16S rRNA gene sequence similarity, this strain was identified as a member of the , most closely related to an uncultured member of the as well as two cultured organisms, sp. L32 and sp. SC2. This strain differed from extant species in cell shape, size, expression of soluble methane monooxygenase and its unique spiny surface layers, composed of polysaccharide. DNA–DNA hybridization results showed only 3.8 % relatedness with NCIMB 13100 and 41.1 % relatedness with SV97. Based on these genotypic and physiological differences, this isolate is proposed as a member of a novel species of the genus , sp. nov. (type strain CSC1 =ATCC BAA-1344 =DSM 18500).

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2007-08-01
2021-04-22
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