1887

Abstract

Four bacterial strains were isolated from independent clinical specimens in different countries and their genotypic and phenotypic characters support their classification in a novel species within the genus . One strain was clearly responsible for a severe, post-traumatic wound infection in a healthy boy. The novel species, for which the name sp. nov. is proposed, is yellow-pigmented, non-photochromogenic and grows in less than a week on solid medium. Based on phenotypic investigations alone, distinction of these four strains from known scotochromogenic rapidly growing strains is problematic. However, the novel strains differ from any other mycobacterium in each of the molecular species markers investigated: the 16S rRNA gene, the 16S–23S rRNA gene internal transcribed spacer and the gene. Of the strains investigated, two different sequevars were detected for the region. Phylogenetic analysis revealed that these four strains were most closely related to . The type strain of sp. nov. is B9-21-178 (=DSM 44395=CIP 109237).

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2006-11-01
2020-01-28
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References

  1. Adékambi, T., Berger, P., Raoult, D. & Drancourt, M. ( 2006; ). rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bollettii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int J Syst Evol Microbiol 56, 133–143.[CrossRef]
    [Google Scholar]
  2. Brown-Elliott, B. A. & Wallace, R. J., Jr ( 2002; ). Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria. Clin Microbiol Rev 15, 716–746.[CrossRef]
    [Google Scholar]
  3. Butler, W. R. & Kilburn, J. O. ( 1988; ). Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids. J Clin Microbiol 26, 50–53.
    [Google Scholar]
  4. Kent, P. T. & Kubica, G. P. ( 1985; ). Public Health Mycobacteriology. A Guide for the Level III Laboratory. Atlanta: US Department of Health and Human Services.
  5. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  6. McNabb, A., Eisler, D., Adie, K., Amos, M., Rodrigues, M., Stephens, G., Black, W. A. & Isaac-Renton, J. ( 2004; ). Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of mycobacterium species isolated from clinical sources. J Clin Microbiol 42, 3000–3011.[CrossRef]
    [Google Scholar]
  7. NCCL ( 2002; ). Susceptibility testing for Mycobacteria, Nocardiae and other aerobic Actinomycetes. Approved standard M24-A. Wayne, PA: National Committee for Clinical Laboratory Standards.
  8. Reischl, U., Emler, S., Horak, Z., Kaustova, J., Kroppenstedt, R. M., Lehn, N. & Naumann, L. ( 1998; ). Mycobacterium bohemicum sp. nov., a new slow-growing scotochromogenic mycobacterium. Int J Syst Bacteriol 48, 1349–1355.[CrossRef]
    [Google Scholar]
  9. Roth, A., Fischer, M., Hamid, M. E., Michalke, S., Ludwig, W. & Mauch, H. ( 1998; ). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S–23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 36, 139–147.
    [Google Scholar]
  10. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  11. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI.
  12. Schröder, K. H., Neumann, L., Kroppenstedt, R. M. & Reischl, U. ( 1997; ). Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic mycobacterium. Int J Syst Bacteriol 47, 86–91.[CrossRef]
    [Google Scholar]
  13. Takewaki, S., Okuzumi, K., Manabe, I., Tanimura, M., Miyamura, K., Nakahara, K., Yazaki, Y., Ohkubo, A. & Nagai, R. ( 1994; ). Nucleotide sequence comparison of the mycobacterial dnaJ gene and PCR-restriction fragment length polymorphism analysis for identification of mycobacterial species. Int J Syst Bacteriol 44, 159–166.[CrossRef]
    [Google Scholar]
  14. Tortoli, E. ( 2003; ). Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16, 319–354.[CrossRef]
    [Google Scholar]
  15. Wallace, R. J., Jr, Brown-Elliott, B. A., Wilson, R. W. & 14 other authors ( 2004; ). Clinical and laboratory features of Mycobacterium porcinum. J Clin Microbiol 42, 5689–5697.[CrossRef]
    [Google Scholar]
  16. Wolinsky, E. ( 1979; ). Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis 119, 107–159.
    [Google Scholar]
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Fatty acid methyl ester content of strain B9-21-178 and related mycobacterial species. [PDF](27 KB)

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Phylogenetic tree based on gene sequences of selected mycobacterial species. [PDF](102 KB)

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