1887

Abstract

A Gram-negative, rod-shaped bacterium, strain 3-2W4, was isolated from the aeration tank of a wastewater treatment plant in Zurich and was found to have the exceptional capacity to degrade synthetic -peptides. 16S rRNA gene sequence analysis showed that strain 3-2W4 is closely related to Y2, but DNA–DNA hybridization experiments between these two strains revealed that they belong to two different species. The two strains displayed different fingerprints after PCR analysis using the repetitive primers BOX, ERIC and REP. Strain 3-2W4 did not degrade microcystin, which is a characteristic trait of Y2. Like Y2, strain 3-2W4 had the following characteristics: fatty acids comprising mainly C , summed feature 3 (C and/or iso-C 2-OH) and C, the presence of ubiquinone Q-10 and -homospermidine as the predominant polyamine compound. The polar lipid profiles of the two strains were almost identical, consisting of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and sphingoglycolipid. Strain 3-2W4 and Y2 utilized the -peptides H-hVal-hAla-hLeu-OH and H-hAla-hLeu-OH as sole carbon and energy sources and shared -peptidyl aminopeptidase activity in common, which distinguishes them from and type strains. On the basis of these results, strain 3-2W4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3-2W4 (=DSM 17130=CCUG 52537). The descriptions of the genus and the species are emended.

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2007-01-01
2020-01-27
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vol. , part 1, pp. 107 - 113

Polar lipid profile of strain 3-2W4 after two-dimensional TLC and detection with molybdatophosphoric acid. DPG, Diphosphatidylglycerol; Lx, unknown polar lipid; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PLx, unknown phospholipid ('x' indicates that the phospolipid does not possess a reference in the polar lipid profiles of species of the as reported by Busse , 1999); PME, phosphatidylmonomethylethanolamine; rPig; red pigment; SGL, sphingoglycolipid.

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Chemotaxonomic characterisation of . , 242–251.



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