1887

Abstract

Strain HHS 31, a Gram-positive, motile, rod-shaped, non-spore-forming, alkaliphilic bacterium, was isolated from the melt water of a glacier. Phenotypic and chemotaxonomic characteristics indicate that strain HHS 31 is related to species of the genus . The 16S rRNA gene sequence similarities between HHS 31 and strains of known species confirm that it is closely related to members of the genus (93–99 %) and that it exhibits >97 % similarity with DSM 20416 (98.9 %), DSM 14480 (98.0 %), JCM 12280 (97.9 %) and DSM 14481 (97.4 %). Phylogenetic analysis based on the 16S rRNA gene sequence further confirms the affiliation of HHS 31 with the genus . However, the levels of DNA–DNA relatedness between HHS 31 and JCM 12280, DSM 20416, DSM 14481 and DSM 14480 are 50, 63, 67 and 28 %, respectively. Strain HHS 31 also differs from these four closely related species in terms of a number of phenotypic traits. The phenotypic, chemotaxonomic and phylogenetic data suggest that HHS 31 merits the status of a novel species, for which the name sp. nov. is proposed. The type strain is HHS 31 (=LMG 23471=IAM 15368).

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2006-12-01
2024-12-08
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References

  1. Chaturvedi P., Reddy G. S. N., Shivaji S. 2005; Dyadobacter hamtensis sp. nov., from Hamta glacier, Himalayas, India. Int J Syst Evol Microbiol 55:2113–2117 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Lund B. M., Farrow J. A. E., Schleifer K. H. 1983; Chemotaxonomic study of an alkalophilic bacterium Exiguobacterium aurantiacum gen. nov. sp. nov.. J Gen Microbiol 129:2037–2042
    [Google Scholar]
  4. Farrow J. A. E., Wallbanks S., Collins M. D. 1994; Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera Caryophanon , Exiguobacterium , Kurthia and Planococcus . Int J Syst Bacteriol 44:74–82 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  6. Fruhling A., Schumann P., Hippe H., Straubler B., Stackebrandt E. 2002; Exiguobacterium undae sp. nov. and Exiguobacterium antarcticum sp. nov. Int J Syst Evol Microbiol 52:1171–1176 [CrossRef]
    [Google Scholar]
  7. Gee J. M., Lund B. M., Metcalf G., Peel J. L. 1980; Properties of a new group of alkalophilic bacteria. J Gen Microbiol 117:9–17
    [Google Scholar]
  8. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  9. Kim I.-G., Lee M.-H., Jung S.-Y., Song J. J., Oh T.-K., Yoon J.-H. 2005; Exiguobacterium aestuarii sp. nov. and Exiguobacterium marinum sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55:885–889 [CrossRef]
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–206
    [Google Scholar]
  12. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  13. Lopez-Cortes A., Schumann P., Pukall R., Stackebrandt E. 2006; Exiguobacterium mexicanum sp. nov. and Exiguobacterium artemiae sp. nov., isolated from the brine shrimp Artemia franciscana . Syst Appl Microbiol 29:183–190 [CrossRef]
    [Google Scholar]
  14. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  15. Reddy G. S. N., Matsumoto G. I., Shivaji S. 2003; Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica. Int J Syst Evol Microbiol 53:1363–1367 [CrossRef]
    [Google Scholar]
  16. Rodrigues D. F., Goris J., Vishnivetskaya T., Gilichinsky D., Thomashaw M. F., Tiedje J. M. 2006; Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov. Extremophiles 10:285–294 [CrossRef]
    [Google Scholar]
  17. Sato N. S., Murata N. 1988; Membrane lipids. Methods Enzymol 167:251–259
    [Google Scholar]
  18. Shivaji S., Shyamala Rao N., Saisree L., Reddy G. S. N., Seshu Kumar G., Bhargava P. M. 1989; Isolates of Arthrobacter from the soils of Schirmacher Oasis, Antarctica. Polar Biol 10:225–229
    [Google Scholar]
  19. Shivaji S., Ray M. K., Shyamala Rao N., Saisree L., Jagannadham M. V., Seshu Kumar G., Reddy G. S. N., Bhargava P. M. 1992; Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42:102–106 [CrossRef]
    [Google Scholar]
  20. Shivaji S., Bhanu V. N., Aggarwal R. K. 2000; Identification of Yersinia pestis as the causative organism of Plague in India as determined by 16S rDNA sequencing and RAPD-based genomic fingerprinting. FEMS Microbiol Lett 189:247–252 [CrossRef]
    [Google Scholar]
  21. Shivaji S., Reddy G. S. N., Raghavan P. U. M., Sarita N. B., Delille D. 2004 Psychrobacter salsus sp. nov. and Psychrobacter adeliensis sp. nov. isolated from fast ice from Adelie Land, Antarctica. Syst Appl Microbiol 27, 628–635 [CrossRef]
  22. Shivaji S., Chaturvedi P., Reddy G. S. N., Suresh K. 2005a; Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55:1083–1088 [CrossRef]
    [Google Scholar]
  23. Shivaji S., Suresh K., Chaturvedi P., Dube S., Sengupta S. 2005b; Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India. Int J Syst Evol Microbiol 55:1123–1127 [CrossRef]
    [Google Scholar]
  24. Shivaji S., Gupta P., Chaturvedi P., Suresh K., Delille D. 2005c; Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55:1453–1456 [CrossRef]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M. 1994; A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  28. Tourova T. P., Antonov A. S. 1987; Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19:333–355
    [Google Scholar]
  29. Yumoto I., Narisawa M. H., Hirota K., Shingyo T., Takebe F., Nodasaka Y., Matsuyama H., Hara I. 2004; Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity. Int J Syst Evol Microbiol 54:2013–2017 [CrossRef]
    [Google Scholar]
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