1887

Abstract

Two bacterial strains, designated GH34-4 and GH41-7, were isolated from greenhouse soil cultivated with cucumber. The bacteria were strictly aerobic, Gram-negative, rod-shaped and oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that these strains belong to the genus within the . Strain GH34-4 showed highest sequence similarity to GH19-3 (97.5 %) and Dae16 (96.4 %), and strain GH41-7 showed highest sequence similarity to DSM 2044 (97.5 %), DSM 2043 (97.5 %) and ATCC 29489 (97.4 %). Levels of DNA–DNA relatedness indicated that strains GH34-4 and GH41-7 represented species clearly different from , , and . The major cellular fatty acids of strains GH34-4 and GH41-7 were iso-C, iso-C and iso-C 9, and the major isoprenoid quinone was Q-8. The DNA G+C contents of GH34-4 and GH41-7 were 62.5 and 66.6 mol%, respectively. On the basis of the polyphasic taxonomic data presented, it is evident that each of these strains represents a novel species of the genus , for which the names sp. nov. (type strain GH34-4=KACC 11587=DSM 18244) and sp. nov. (type strain GH41-7=KACC 11588=DSM 18481) are proposed.

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2007-03-01
2021-03-05
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References

  1. Bae H.-S., Im W.-T., Lee S.-T. 2005; Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 55:1155–1161 [CrossRef]
    [Google Scholar]
  2. Christensen P., Cook F. D. 1978; Lysobacter , a new genus of nonfruiting, gliding bacteria with a high base ratio. Int J Syst Bacteriol 28:367–393 [CrossRef]
    [Google Scholar]
  3. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  4. Hiraishi A. 1992; Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15:210–213 [CrossRef]
    [Google Scholar]
  5. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  6. Lee J. W., Im W.-T., Kim M. K., Yang D.-C. 2006; Lysobacter koreensis sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 56:231–235 [CrossRef]
    [Google Scholar]
  7. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  8. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  9. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  10. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa , Bacillus macerans , Bacillus azotofixans , and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [CrossRef]
    [Google Scholar]
  11. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Swings J., Christensen P. 1989; Genus I. Lysobacter Christensen and Cook 1978, 372AL . In Bergey's Manual of Systematic Bacteriology vol. 3 pp  2083–2089 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  13. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  14. Weon H.-Y., Kim B.-Y., Baek Y.-K., Yoo S.-H., Kwon S.-W., Stackebrandt E., Go S.-J. 2006; Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. Int J Syst Evol Microbiol 56:947–951 [CrossRef]
    [Google Scholar]
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