1887

Abstract

A novel alkaliphilic and psychrophilic bacterium was isolated from the cold and alkaline ikaite tufa columns of the Ikka Fjord in south-west Greenland. According to 16S rRNA gene sequence analysis, strain GCM71 belonged to the family ‘’ in the phylum . Strain GCM71, together with five related isolates from ikaite columns, formed a separate cluster with 86–93 % gene sequence similarity to their closest relative, . The G+C content of the DNA from strain GCM71 was 43.1 mol%, whereas that of was reported to be 35 mol%. DNA–DNA hybridization between strain GCM71 and was 9.5 %. The strain was red pigmented, Gram-negative, strictly aerobic with non-motile, rod-shaped cells. The optimal growth conditions for strain GCM71 were pH 9.2–10.0, 5 °C and 0.6 % NaCl. The fatty acid profile of the novel strain was dominated by branched and unsaturated fatty acids (90–97 %), with a high abundance of iso-C 9 (17.5 %), iso-C 3-OH (17.5 %) and summed feature 3, comprising iso-C 2-OH and/or C 7 (12.6 %). Phylogenetic, chemotaxonomic and physiological characteristics showed that the novel strain could not be affiliated to any known genus. A new genus, gen. nov., is proposed to accommodate the novel strain. Strain GCM71 (=DSM 17998=LMG 23454) is proposed as the type strain of the type species, sp. nov.

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2006-12-01
2021-04-10
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