1887

Abstract

An aerobic, Gram-negative, yellow-pigmented, non-motile bacterium, designated strain KMM 3882, was isolated from a marine bivalve () collected from Peter the Great Bay, Sea of Japan, and was subjected to phenotypic and phylogenetic analyses. Strain KMM 3882 was found to exert a remarkable inhibitory activity against a number of Gram-positive micro-organisms. Phylogenetic analysis based on 16S rRNA gene sequences placed strain KMM 3882 within the genus , as an independent lineage adjacent to DS-4 and DSM 15761. Strain KMM 3882 showed the highest 16S rRNA gene sequence similarity to DS-4 (97.3 %); similarities of 96.5–96.7 % were obtained with DSM 13101, NBRC 15497, NBRC 15499, DSM 7225 and DSM 15761. Chemotaxonomically, strain KMM 3882 contained sphingoglycolipid, C and C as predominant fatty acids and 2-OH C as a major 2-hydroxy fatty acid, confirming the affiliation of strain KMM 3882 with the genus . On the basis of phylogenetic analysis, DNA–DNA hybridization and physiological and biochemical characterization, strain KMM 3882 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KMM 3882 (=An 18=NRIC 0685=JCM 14122=CIP 109223).

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2007-02-01
2019-10-20
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Two-dimensional thin-layer chromatogram of polar lipids of strain KMM 3882 . [PDF](34 KB)

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Phylogenetic tree based on 16S rRNA gene sequences constructed by using maximum-likelihood. [PDF](15 KB)

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Phylogenetic tree based on 16S rRNA gene sequences constructed by using maximum-parsimony. [PDF](15 KB)

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Antimicrobial properties of butanol extract from cells of strain KMM 3882 and cellular fatty acid profiles of strain KMM 3882 and type strains of related species. [PDF](27 KB)

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