A Gram-negative, aerobic, motile, rod-shaped bacterium (strain Gsoil 250) was isolated from soil of a ginseng field in Pocheon province (South Korea) and was characterized using a polyphasic approach in order to determine its taxonomic position. Comparative analysis of 16S rRNA gene sequences showed that strain Gsoil 250 belonged to the family of the class and was related to (98.7 %), (98.2 %), (97.9 %), (97.9 %) and (97.8 %). The phylogenetic distance from any other species with validly published names within the genus was greater than 3.8 %. The G+C content of the genomic DNA of strain Gsoil 250 was 69.2 mol%. Strain Gsoil 250 contained Q-10 as the predominant respiratory lipoquinone and C 7 and summed feature 4 (C 7 and/or iso-C 2-OH; 34.6 %) as the major fatty acids. No 3-hydroxy fatty acids were detected. Major polar lipids consisted of sphingoglycolipid, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol and two unknown glycolipids. Phenotypic and chemotaxonomic data supported the affiliation of strain Gsoil 250 to the genus . The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Gsoil 250 from the eight recognized species. Strain Gsoil 250 therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain Gsoil 250 (=KCTC 12582=LMG 23390).


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Transmission electron micrograph of a negatively stained single cell of strain Gsoil 250 ( sp. nov.). [PDF](29 KB)


Two-dimensional, thin-layer chromatographs of the polar lipids of strain Gsoil 250 ( sp. nov.). [PDF](169 KB)


Cellular fatty acid profiles of strain Gsoil 250 ( sp. nov.) and type strains of related species of the genus . [PDF](49 KB)


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