1887

Abstract

Rogosa . 1953 was described as a homofermentative lactobacillus with two varieties: , typified inter alia by the ability to ferment rhamnose, and , characterized by the ability to ferment the glucoside salicin. These varieties have become accepted as subspecies divisions. We have examined the relatedness of 32 strains by a polyphasic approach. Carbohydrate fermentation profile analysis did not support clear distinction of the two subspecies. UCC118 was shown to be facultatively heterofermentative, confirming genome analysis. 16S rRNA gene sequences and 16S–23S rRNA intergenic spacer region sequences provided no discrimination between any of the strains or subspecies. Broad subdivisions were distinguishable by pulsed-field gel genomic digest patterns, but they did not allow subspecific or phenotypic distinctions. A phylogeny based upon gene sequences was discordant with rhamnose or salicin fermentation data for many taxa, and no reliable phenotypic correlations could be established. In the absence of meaningful taxonomic criteria, we therefore propose that comprises a single species with no infraspecific taxa. Based on the present study and literature data, an emended description of the species is provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64426-0
2006-10-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/10/2397.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64426-0&mimeType=html&fmt=ahah

References

  1. Ahrne, S., Nobaek, S., Jeppsson, B., Adlerberth, I., Wold, A. E. & Molin, G. ( 1998; ). The normal Lactobacillus flora of healthy human rectal and oral mucosa. J Appl Microbiol 85, 88–94.[CrossRef]
    [Google Scholar]
  2. Badet, M. C., Richard, B. & Dorignac, G. ( 2001; ). An in vitro study of the pH-lowering potential of salivary lactobacilli associated with dental caries. J Appl Microbiol 90, 1015–1018.[CrossRef]
    [Google Scholar]
  3. Bringel, F., Castioni, A., Olukoya, D. K., Felis, G. E., Torriani, S. & Dellaglio, F. ( 2005; ). Lactobacillus plantarum subsp. argentoratensis subsp. nov., isolated from vegetable matrices. Int J Syst Evol Microbiol 55, 1629–1634.[CrossRef]
    [Google Scholar]
  4. Brousseau, R., Hill, J. E., Prefontaine, G., Goh, S. H., Harel, J. & Hemmingsen, S. M. ( 2001; ). Streptococcus suis serotypes characterized by analysis of chaperonin 60 gene sequences. Appl Environ Microbiol 67, 4828–4833.[CrossRef]
    [Google Scholar]
  5. Claesson, M. J., Li, Y., Leahy, S. & 12 other authors ( 2006; ). Multireplicon genome architecture of Lactobacillus salivarius. Proc Natl Acad Sci U S A 103, 6718–6723.[CrossRef]
    [Google Scholar]
  6. Dellaglio, F., Felis, G. E., Castioni, A., Torriani, S. & Germond, J. E. ( 2005; ). Lactobacillus delbrueckii subsp. indicus subsp. nov., isolated from Indian dairy products. Int J Syst Evol Microbiol 55, 401–404.[CrossRef]
    [Google Scholar]
  7. Dunne, C., Murphy, L., Flynn, S. & 12 other authors ( 1999; ). Probiotics: from myth to reality. Demonstration of functionality in animal models of disease and in human clinical trials. Antonie van Leeuwenhoek 76, 279–292.[CrossRef]
    [Google Scholar]
  8. Flynn, S., van Sinderen, D., Thornton, G. M., Holo, H., Nes, I. F. & Collins, J. K. ( 2002; ). Characterization of the genetic locus responsible for the production of ABP-118, a novel bacteriocin produced by the probiotic bacterium Lactobacillus salivarius subsp. salivarius UCC118. Microbiology 148, 973–984.
    [Google Scholar]
  9. Fujisawa, T. & Mitsuoka, T. ( 1996; ). Homofermentative Lactobacillus species predominantly isolated from canine feces. J Vet Med Sci 58, 591–593.[CrossRef]
    [Google Scholar]
  10. Guindon, S. & Gascuel, O. ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef]
    [Google Scholar]
  11. Gurtler, V. & Stanisich, V. A. ( 1996; ). New approaches to typing and identification of bacteria using the 16S–23S rDNA spacer region. Microbiology 142, 3–16.[CrossRef]
    [Google Scholar]
  12. Heilig, H. G., Zoetendal, E. G., Vaughan, E. E., Marteau, P., Akkermans, A. D. & de Vos, W. M. ( 2002; ). Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol 68, 114–123.[CrossRef]
    [Google Scholar]
  13. Hung, W. C., Tsai, J. C., Hsueh, P. R., Chia, J. S. & Teng, L. J. ( 2005; ). Species identification of mutans streptococci by groESL gene sequence. J Med Microbiol 54, 857–862.[CrossRef]
    [Google Scholar]
  14. Kandler, O. ( 1983; ). Carbohydrate metabolism in lactic acid bacteria. Antonie van Leeuwenhoek 49, 209–224.[CrossRef]
    [Google Scholar]
  15. Kandler, O. & Weiss, N. ( 1986; ). Regular, nonsporing Gram-positive rods. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1208–1209. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Koll-Klais, P., Mandar, R., Leibur, E., Marcotte, H., Hammarstrom, L. & Mikelsaar, M. ( 2005; ). Oral lactobacilli in chronic periodontitis and periodontal health: species composition and antimicrobial activity. Oral Microbiol Immunol 20, 354–361.[CrossRef]
    [Google Scholar]
  17. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  18. Lapage, S. P., Sneath, P. H. A., Lessel, E. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors) ( 1992; ). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  19. London, J. ( 1976; ). The ecology and taxonomic status of the lactobacilli. Annu Rev Microbiol 30, 279–301.[CrossRef]
    [Google Scholar]
  20. Mitsuoka, T. ( 1969; ). Comparative studies on lactobacilli from the faeces of man, swine and chickens. Zentralbl Bakteriol Orig 210, 32–51 (in German).
    [Google Scholar]
  21. Molin, G., Jeppsson, B., Johansson, M. L., Ahrne, S., Nobaek, S., Stahl, M. & Bengmark, S. ( 1993; ). Numerical taxonomy of Lactobacillus spp. associated with healthy and diseased mucosa of the human intestines. J Appl Bacteriol 74, 314–323.[CrossRef]
    [Google Scholar]
  22. Rogosa, M. & Sharpe, M. E. ( 1959; ). An approach to the classification of the lactobacilli. J Appl Bacteriol 22, 329–340.
    [Google Scholar]
  23. Rogosa, M., Wiseman, R. F., Mitchell, J. A., Disraely, M. N. & Beaman, A. J. ( 1953; ). Species differentiation of oral lactobacilli from man including description of Lactobacillus salivarius nov. spec. and Lactobacillus cellobiosus nov. spec. J Bacteriol 65, 681–699.
    [Google Scholar]
  24. Skerman, V. B. D., McGowan, V. & Sneath, P. H. A. ( 1980; ). Approved lists of bacterial names. Int J Syst Bacteriol 30, 225–420.[CrossRef]
    [Google Scholar]
  25. Song, Y., Kato, N., Liu, C., Matsumiya, Y., Kato, H. & Watanabe, K. ( 2000; ). Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiol Lett 187, 167–173.
    [Google Scholar]
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  27. Tsai, J. C., Hsueh, P. R., Lin, H. M., Chang, H. J., Ho, S. W. & Teng, L. J. ( 2005; ). Identification of clinically relevant Enterococcus species by direct sequencing of groES and spacer region. J Clin Microbiol 43, 235–241.[CrossRef]
    [Google Scholar]
  28. Ventura, M., Canchaya, C., Zink, R., Fitzgerald, G. F. & van Sinderen, D. ( 2004; ). Characterization of the groEL and groES loci in Bifidobacterium breve UCC 2003: genetic, transcriptional, and phylogenetic analyses. Appl Environ Microbiol 70, 6197–6209.[CrossRef]
    [Google Scholar]
  29. Woo, P. C., Fung, A. M., Lau, S. K. & Yuen, K. Y. ( 2002; ). Identification by 16S rRNA gene sequencing of Lactobacillus salivarius bacteremic cholecystitis. J Clin Microbiol 40, 265–267.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64426-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64426-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error