1887

Abstract

A novel yeast strain, CBS 10258, was isolated from the atmosphere in central South Africa. Sequence analysis of the D1/D2 domain and internal transcribed spacer region of the novel strain indicates that it represents a novel species within the complex. Phylogenetic analyses based on the D1/D2 domain revealed that the novel strain occupies a relatively isolated position within this complex with , , and sp. CBS 8363 being the closest relatives. However, the novel strain could be distinguished from related species by standard physiological tests including the inability to assimilate rhamnose, methyl --glucoside, salicin, lactose, erythritol, ribitol, xylitol, citrate and ethanol. In addition, no extracellular starch production was observed and the isolate was able to grow in the absence of additional vitamins. On the basis of these results, we suggest that the new strain represents a novel species for which the name sp. nov. is proposed [type strain CBS 10258 (=NRRL Y-27920)].

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2006-11-01
2024-04-27
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References

  1. Ellis D. H., Pfeiffer T. J. 1990; Ecology, life cycle and infectious propagule of Cryptococcus neoformans . Lancet 336:923–925 [CrossRef]
    [Google Scholar]
  2. Fell J. W., Statzell-Tallman A. 1998; Cryptococcus Vuillemin. In The Yeasts, a Taxonomic Study , 4th edn. pp  742–1767 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
  3. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [CrossRef]
    [Google Scholar]
  4. Fortes S. T., Lazera M. S., Nishikawa M. M., Macedo R. C., Wanke B. 2001; First isolation of Cryptococcus neoformans var. gattii from a native jungle tree in the Brazilian Amazon rainforest. Mycoses 44:137–140 [CrossRef]
    [Google Scholar]
  5. Golubev W. I., Gadanho M., Sampaio J. P., Golubev N. W. 2003; Cryptococcus nemorosus sp. nov. and Cryptococcus perniciosus sp. nov., related to Papiliotrema Sampaio et al . (Tremellales). Int J Syst Evol Microbiol 53:905–911 [CrossRef]
    [Google Scholar]
  6. Granados D. P., Castaneda E. 2005; Isolation and characterization of Cryptococcus neoformans varieties recovered from natural sources in Bogota, Colombia, and study of ecological conditions in the area. Microb Ecol 49:282–290 [CrossRef]
    [Google Scholar]
  7. Kumar S., Tamura K., Nei M. 2004; mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  8. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [CrossRef]
    [Google Scholar]
  9. Randhawa H. S., Mussa A. Y., Khan Z. U. 2001; Decaying wood in tree trunk hollows as a natural substrate for Cryptococcus neoformans and other yeast-like fungi of clinical interest. Mycopathologia 151:63–69 [CrossRef]
    [Google Scholar]
  10. Ruiz A., Bulmer G. S. 1981; Particle size of airborne Cryptococcus neoformans in a tower. Appl Environ Microbiol 41:1225–1229
    [Google Scholar]
  11. Sampaio J. P., Weiss M., Gananho M., Bauer R. 2002; New taxa in the Tremellales: Bulleribasidium oberjochense gen.et sp. nov., Papiliotrema bandonii gen. et sp.nov. and Fibulobasidium murrhardtense sp. nov.. Mycologia 94873–887 [CrossRef]
    [Google Scholar]
  12. Scorzetti G., Fell J. W., Fonseca A., Statzell-Tallman A. 2002; Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 14971–23
    [Google Scholar]
  13. Sorrell T. C., Ellis D. H. 1997; Ecology of Cryptococcus neoformans . Rev Iberoam Micol 14:42–43
    [Google Scholar]
  14. Sugita T., Takashima M., Ikeda R., Nakase T., Shinoda T. 2000; Intraspecies diversity of Cryptococcus laurentii as revealed by sequences of internal transcribed spacer regions and 28S rRNA gene and taxonomic position of C. laurentii clinical isolates. J Clin Microbiol 38:1468–1471
    [Google Scholar]
  15. Sukroongreung S., Eampokalap B., Tansuphaswadikul S., Nilakul C., Tantimavanich S. 1999; Recovery of Cryptococcus neoformans from the nasopharynx of AIDS patients. Mycopathologia 143:131–134
    [Google Scholar]
  16. Takashima M., Sugita T., Shinoda T., Nakase T. 2003; Three new combinations from the Cryptococcus laurentii complex: Cryptococcus aureus , Cryptococcus carnescens and Cryptococcus peneaus . Int J Syst Evol Microbiol 53:1187–1194 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  18. Van Wyk P. W. J., Wingfield M. J. 1994; Ultrastructure of ascus arrangement and ascospore development in Ophiostoma seticolle . Mycologia 86:607–614 [CrossRef]
    [Google Scholar]
  19. White T. J., Bruns T., Lee S., Taylor J. W. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications pp  315–322 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. San Diego, CA: Academic Press;
    [Google Scholar]
  20. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study , 4th edn. pp  77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
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