1887

Abstract

A Gram-negative, aerobic, halophilic, neutrophilic, rod-shaped, non-pigmented, polar-flagellated bacterium, UST010306-043, was isolated from a pearl-oyster culture pond in Sanya, Hainan Province, China in January 2001. This marine bacterium had an optimum temperature for growth of between 33 and 37 °C. On the basis of 16S rRNA gene sequence analysis, the strain was closely related to and , with 97.5–97.7 and 97.1 % sequence similarity, respectively. Levels of DNA–DNA relatedness to the type strains of these species were well below 70 %. Analyses of phylogenetic, phenotypic and chemotaxomonic characteristics showed that strain UST010306-043 was distinct from currently established species. A novel species with the name sp. nov. is proposed to accommodate this bacterium, with strain UST010306-043 (=JCM 13672=NRRL B-41433) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64392-0
2006-10-01
2020-07-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/10/2271.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64392-0&mimeType=html&fmt=ahah

References

  1. Amann R. I., Lin C., Key R., Montgomery L., Stahl D. A. 1992; Diversity among Fibrobacter strains: towards a phylogenetic classification. Syst Appl Microbiol 15:23–31
    [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine gram-negative eubacteria: genera Photobacterium , Beneckea, Alteromonas Pseudomonas and Alcaligenes . In The Prokaryotes , vol. 1 pp  1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. Berlin: Springer;
    [Google Scholar]
  3. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 3:402–429
    [Google Scholar]
  4. Brown T. 2005; Dot and slot blotting of DNA onto a positively charged nylon membrane using a manifold. In Current Protocols in Molecular Biology pp. 2.9.18–2.9.20 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  5. Collins C. H., Lyne P. M., Grange J. M. 1989 Collins and Lyne's Microbiological Methods London: Butterworth;
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  8. Ivanova E. P., Zhukova N. V., Svetashev V. I., Gorshkova N. M., Kurilenko V. V., Frolova G. M., Mikhailov V. V. 2000; Evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of Alteromonas -like proteobacteria. Curr Microbiol 41:341–345 [CrossRef]
    [Google Scholar]
  9. Ivanova E. P., Onyshchenko O. M., Christen R., Lysenko A. M., Zhukova N. V., Shevchenko L. S., Kiprianova E. A. 2005; Marinomonas pontica sp. nov., isolated from the Black Sea. Int J Syst Evol Microbiol 55:275–279 [CrossRef]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  12. Lau K. W. K., Ng C. Y. M., Ren J., Lau S. C. L., Qian P.-Y., Wong P.-K., Lau T. C., Wu M. 2005; Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacterium of the phylum ‘ Bacteroidetes ’. Int J Syst Evol Microbiol 55, 1051–1057 [CrossRef]
  13. Lewin R. A., Lounsbery D. M. 1969; Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58:145–170 [CrossRef]
    [Google Scholar]
  14. Macián M. C., Arahal D. R., Garay E., Pujalte M. J. 2005; Marinomonas aquamarina sp. nov., isolated from oysters and seawater. Syst Appl Microbiol 28:145–150 [CrossRef]
    [Google Scholar]
  15. Maloy S. R. 1990 Experimental Techniques in Bacterial Genetics Boston: Jones and Bartlett;
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  17. Muyzer G., Teske A., Wirsen C. O., Jannasch H. W. 1995; Phylogenetic relationships of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments. Arch Microbiol 164:165–172 [CrossRef]
    [Google Scholar]
  18. Prabagaran S. R., Suresh K., Manorama R., Delille D., Shivaji S. 2005; Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica. Int J Syst Evol Microbiol 55:309–313 [CrossRef]
    [Google Scholar]
  19. Romanenko L. A., Uchino M., Mikhailov V. V., Zhukova N. V., Uchimura T. 2003; Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53:829–832 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Sanchez-Amat A., Solano F. 2005; Genus III. Marinomonas Van Landschoot and De Ley 1984, 91VP (effective publication: Van Landschoot and De Ley 1983, 3071. In Bergey's Manual of Systematic Bacteriology , vol. 2, part B pp  284–289 Edited by Brenner D. J., Krieg N. R., Staley J. T. New York: Springer;
    [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  23. Solano F., Sanchez-Amat A. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49:1241–6 [CrossRef]
    [Google Scholar]
  24. Solano F., García E., Perez De Egea E., Sanchez-Amat A. 1997; Isolation and characterization of strain MMB-1 (CECT 4803), a novel melanogenic marine bacterium. Appl Environ Microbiol 63:3499–3506
    [Google Scholar]
  25. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  26. Svetashev V. I., Vysotskii M. V., Ivanova E. P., Mikhailov V. V. 1995; Cellular fatty acids of Alteromonas species. Syst Appl Microbiol 18:37–43 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  28. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas , Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  30. Yoon J.-H., Kang S.-J., Oh T.-K. 2005; Marinomonas dokdonensis sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55:2303–2307 [CrossRef]
    [Google Scholar]
  31. Zhang Z., Schwartz S., Wagner L., Miller W. 2000; A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203–214 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64392-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64392-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error