1887

Abstract

Halophilic archaeal strains ABH32 and AX-7 were isolated from Aibi salt lake in Xin-Jiang, China, and were subjected to taxonomic studies. The cells of both strains were rod-shaped, and growth required at least 10 % (w/v) NaCl. The cellular polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1→2)-glucose glycerol diether (S-DGD). The isolates were different in that strain ABH32 did not require magnesium and had strong ability to assimilate sugars for growth, whereas strain AX-7 required magnesium and did not assimilate glucose, sucrose or maltose for growth. Both strain ABH32 and strain AX-7 were most closely related to members of the genera and . On the basis of phenotypic and genotypic characteristics, especially their cellular polar lipids, the two isolates were assigned to the genus . Moreover, the DNA relatedness values for strains ABH32 and AX-7 with respect to members of the genus were found to be well below 70 %. Thus, strains ABH32 and AX-7 represent two novel species within the genus , for which the names sp. nov. (type strain ABH32=CGMCC 1.5334=JCM 13562) and sp. nov. (type strain AX-7=CGMCC 1.5333=JCM 13563) are proposed.

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2006-08-01
2020-08-04
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References

  1. Cui H.-L., Yang Y., Tothy D., Zhou P.-J., Liu S.-J. 2006; Biodiversity of halophilic Archaea isolated from two salt lakes in Xin-Jiang region of China. Acta Microbiol Sin 46:171–176
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  3. Feng J., Zhou P.-J., Liu S.-J. 2004; Halorubrum xinjiangense sp. nov., a novel halophile isolated from saline lakes in China. Int J Syst Evol Microbiol 54:1789–1791 [CrossRef]
    [Google Scholar]
  4. Feng J., Zhou P.-J., Liu S.-J., Warren-Rhodes K. 2005; Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55:149–152 [CrossRef]
    [Google Scholar]
  5. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  6. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  7. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  8. McGenity T. J., Gemmell R. T., Grant W. D. 1998; Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom.nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48:1187–1196 [CrossRef]
    [Google Scholar]
  9. Montalvo-Rodríguez R., Lopez-Garriga J., Vreeland R. H., Oren A., Ventosa A., Kamekura M. 2000; Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico. Int J Syst Evol Microbiol 50:1065–1071 [CrossRef]
    [Google Scholar]
  10. Oren A., Ventosa A., Grant W. D. 1997; Proposal of minimal standards for the description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  11. Tindall B. J. 2003; Taxonomic problems arising in the genera Haloterrigena and Natrinema . Int J Syst Evol Microbiol 53:1697–1698 [CrossRef]
    [Google Scholar]
  12. Ventosa A., Gutierrez M. C., Kamekura M., Dyall-Smith M. L. 1999; Proposal to transfer Halococcus turkmenicus , Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49:131–136 [CrossRef]
    [Google Scholar]
  13. Xin H.-W., Itoh T., Zhou P.-J., Suzuki K., Kamekura M., Nakase T. 2000; Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xin-Jiang, China. Int J Syst Evol Microbiol 50:1297–1303 [CrossRef]
    [Google Scholar]
  14. Xu X.-W., Liu S.-J., Tothy D., Oren A., Wu M., Zhou P.-J. 2005; Haloterrigena saccharevitans sp. nov., an extremely halophilic archaeon from Xin-Jiang, China. Int J Syst Evol Microbiol 55:2539–2542 [CrossRef]
    [Google Scholar]
  15. Zvyagintseva I. S., Tarasov A. L. 1987; Extreme halophilic bacteria from saline soils. Microbiology (English translation of Mikrobiologiia ) 56664–669
    [Google Scholar]
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