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Abstract

Halophilic archaeal strains ABH32 and AX-7 were isolated from Aibi salt lake in Xin-Jiang, China, and were subjected to taxonomic studies. The cells of both strains were rod-shaped, and growth required at least 10 % (w/v) NaCl. The cellular polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1→2)-glucose glycerol diether (S-DGD). The isolates were different in that strain ABH32 did not require magnesium and had strong ability to assimilate sugars for growth, whereas strain AX-7 required magnesium and did not assimilate glucose, sucrose or maltose for growth. Both strain ABH32 and strain AX-7 were most closely related to members of the genera and . On the basis of phenotypic and genotypic characteristics, especially their cellular polar lipids, the two isolates were assigned to the genus . Moreover, the DNA relatedness values for strains ABH32 and AX-7 with respect to members of the genus were found to be well below 70 %. Thus, strains ABH32 and AX-7 represent two novel species within the genus , for which the names sp. nov. (type strain ABH32=CGMCC 1.5334=JCM 13562) and sp. nov. (type strain AX-7=CGMCC 1.5333=JCM 13563) are proposed.

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2006-08-01
2019-10-22
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Supplements

vol. , part 8, pp. 1837 - 1840

Thin-layer chromatogram on a Merck silica gel 60 F aluminium-backed plate of total polar lipids from strains ABH-32 and AX-7 and reference strains.

Thin-layer chromatogram on a Merck silica gel 60 F aluminium-backed plate of phospholipids of strains ABH-32 and AX-7 , as well as reference strains

[PDF file of Supplementary Figs S1 and S2](103 KB)



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