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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 12

gen. nov., sp. nov., an anaerobic, alkalitolerant, sulfite-reducing bacterium isolated from a district heating plant Free

Abstract

A novel alkalitolerant, anaerobic bacterium, designated strain sk.kt5, was isolated from a metal coupon retrieved from a corrosion-monitoring reactor of a Danish district heating plant (Skanderborg, Jutland). The cells of strain sk.kt5 were motile, rod-shaped (0.4–0.6×2.5–9.6 μm), stained Gram-positive and formed endospores. Strain sk.kt5 grew at pH 7.6–10.5 (with optimum growth at pH 8.0–9.5), at temperatures in the range 23–44 °C (with optimum growth at 35–37 °C), at NaCl concentrations in the range 0–5 % (w/v) (with optimum growth at 0–0.5 %) and required yeast extract for growth. Only a limited number of substrates were utilized as electron donors, including betaine, formate, lactate, methanol, choline and pyruvate. Elemental sulfur, sulfite, thiosulfate, nitrate and nitrite, but not sulfate or Fe(III) citrate, were used as electron acceptors. The G+C content of the DNA was 41.6 mol%. Phylogenetic analyses of the sequence data for the genes [encoding the major subunits of dissimilatory (bi)sulfite reductase] and the 16S rRNA gene placed strain sk.kt5 within a novel lineage in the class of the phylum . Taken together, the physiological and genotypic data suggest that strain sk.kt5 represents a novel species within a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is sk.kt5 (=JCM 12761=DSM 16504).

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2006-12-01
2025-01-21
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Desulfitibacter alkalitolerans gen. nov., sp. nov., an anaerobic, alkalitolerant, sulfite-reducing bacterium isolated from a district heating plant
Int J Syst Evol Microbiol 56, 2831 (2006); https://doi.org/10.1099/ijs.0.64356-0
/content/journal/ijsem/10.1099/ijs.0.64356-0
/content/journal/ijsem/10.1099/ijs.0.64356-0
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