1887

Abstract

A novel black yeast-like fungus, , is described based on two closely related isolates from biological soil crust (BSC) samples collected on the Colorado Plateau (Utah) and in the Great Basin desert (Oregon), USA. Their morphology places them in the anamorphic genus , having affinities to the family (Ascomycota). Phylogenetic analysis of their D1/D2 large subunit nuclear ribosomal RNA (LSU nrRNA) gene sequences suggests that they represent a distinct species. The closest known putative relative to is Samuels, isolated from decorticated wood in Westland County, New Zealand. The holotype for anam. nov. is UAMH 10686 and the type strain is CP141b (=ATCC MYA-3639=CBS 119970=DSM 16793). Dark-pigmented fungi appear to constitute an important heterotrophic component of soil crusts and represents the first description of a dematiaceous fungus isolated from BSCs.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64332-0
2006-11-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/11/2697.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64332-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. J., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Belnap, J. & Gardner, J. S. ( 1993; ). Soil microstructure in soils of the Colorado Plateau: the role of the cyanobacterium Microcoleus vaginatus. Great Basin Nat 53, 40–47.
    [Google Scholar]
  3. Belnap, J., Büdel, B. & Lange, O. L. ( 2001; ). Biological soil crusts: characteristics and distribution. In Biological Soil Crusts: Structure, Function, and Management (Ecological Studies no. 150), pp. 3–30. Edited by J. Belnap & O. L. Lange. Berlin: Springer.
  4. de Hoog, G. S. ( 1993; ). Evolution of black yeasts: possible adaptation to the human host. Antonie van Leeuwenhoek 63, 105–109.[CrossRef]
    [Google Scholar]
  5. de Hoog, G. S. & Hermanides-Nijhof, E. J. ( 1977; ). Survey of black yeasts and allied Hyphomycetes. Stud Mycol 15, 178–221.
    [Google Scholar]
  6. de Hoog, G. S., Vicente, V., Caligiorne, R. B., Kantarcioglu, S., Tintelnot, K., Gerrits van den Ende, A. H. G. & Haase, G. ( 2003; ). Species diversity and polymorphism in the Exophiala spinifera clade containing opportunistic black yeast-like fungi. J Clin Microbiol 41, 4767–4778.[CrossRef]
    [Google Scholar]
  7. Fletcher, J. E. & Martin, W. P. ( 1948; ). Some effects of algae and molds in the rain-crust of desert soils. Ecology 29, 95–100.[CrossRef]
    [Google Scholar]
  8. Galun, M. & Garty, J. ( 2001; ). Biological soil crusts of the Middle East. In Biological Soil Crusts: Structure, Function, and Management (Ecological Studies no. 150), pp. 95–106. Edited by J. Belnap & O. L. Lange. Berlin: Springer.
  9. Garcia-Pichel, F. ( 2002; ). Desert environments: biological soil crusts. In Encyclopedia of Environmental Microbiology, pp. 1019–1023. Edited by G. Bitton. New York: Wiley.
  10. Garcia-Pichel, F., López-Cortés, A. & Nübel, U. ( 2001; ). Phylogenetic and morphological diversity of cyanobacteria in soil desert crusts from the Colorado Plateau. Appl Environ Microbiol 67, 1902–1910.[CrossRef]
    [Google Scholar]
  11. Greuter, W., Mcneill, J., Barrie, F. R. & 9 other authors ( 2000; ). International Code of Botanical Nomenclature (Saint Louis Code). Regnum Vegetabile series, volume 138. Königstein, Germany: Koeltz Scientific Books.
  12. Grishkan, I., Zaady, E. & Nevo, E. ( 2006; ). Soil crust microfungi along a southward rainfall gradient in a desert ecosystems. Eur J Soil Biol 43, 33–42.
    [Google Scholar]
  13. Gundlapally, S. R. & Garcia-Pichel, F. ( 2006; ). The community and phylogenetic diversity of biological soil crusts in the Colorado Plateau studied by molecular fingerprinting and intensive cultivation. Microb Ecol 52, 345–357.[CrossRef]
    [Google Scholar]
  14. Haase, G., Sonntag, L., Melzer-Krick, B. & de Hoog, G. S. ( 1999; ). Phylogenetic inference by SSU-gene analysis of members of the Herpotrichiellaceae with special reference to human pathogenic species. Stud Mycol 43, 80–97.
    [Google Scholar]
  15. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  16. Kirk, P. M., Cannon, P. F., David, J. C. & Stalpers, J. A. ( 2001; ). Ainsworth and Bisby's Dictionary of the Fungi, 9th edn. Wallingford: CABI Publishing.
  17. Kornerup, A. & Wanscher, J. H. ( 1967; ). Methuen Handbook of Colour, 2nd edn. London: Methuen.
  18. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  19. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  20. Lorch, H. J., Benckiser, G. & Ottow, J. C. G. ( 1995; ). Basic methods for counting microorganisms in soil and water. In Methods in Applied Soil Microbiology and Biochemistry, pp. 162–170. Edited by K. Alef & P. Nannipieri. San Diego: Academic Press.
  21. Müller, E., Petrini, O., Fischer, P. J., Samuels, G. J. & Rossman, A. Y. ( 1987; ). Taxonomy and anamorphs of the Herpotrichiellaceae with notes on generic synonymy. T Brit Mycol Soc 88, 63–74.[CrossRef]
    [Google Scholar]
  22. Nagy, M., Pérez, A. & Garcia-Pichel, F. ( 2005; ). The prokaryotic diversity of biological soil crusts in the Sonoran desert (Organ Pipe Cactus National Monument, AZ). FEMS Microbiol Ecol 54, 233–245.[CrossRef]
    [Google Scholar]
  23. O'Donnell, K. ( 1993; ). Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics, pp. 225–233. Edited by D. R. Reynolds & J. W. Taylor. Wallingford: CABI Publishing.
  24. Peterson, S. W. & Kurtzman, C. P. ( 1991; ). Ribosomal RNA sequence divergence among sibling species of yeasts. Syst Appl Microbiol 14, 124–129.[CrossRef]
    [Google Scholar]
  25. Reddy, G. S. N. & Garcia-Pichel, F. ( 2005; ). Dyadobacter crusticola sp. nov., from biological soil crusts in the Colorado Plateau, USA, and an emended description of the genus Dyadobacter Chelius and Triplett 2000. Int J Syst Evol Microbiol 55, 1295–1299.[CrossRef]
    [Google Scholar]
  26. Reddy, G. S. N., Nagy, M. & Garcia-Pichel, F. ( 2006; ). Belnapia moabensis gen. nov., sp. nov., an alphaproteobacterium from biological soil crusts in the Colorado Plateau, USA. Int J Syst Evol Microbiol 56, 51–58.[CrossRef]
    [Google Scholar]
  27. Rippka, R., Deruelles, J., Waterbury, J. B., Herdmann, M. & Stanier, R. Y. ( 1979; ). Generic assignment, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111, 1–16.[CrossRef]
    [Google Scholar]
  28. Smith, S. M., Abed, R. M. M. & Garcia-Pichel, F. ( 2004; ). Biological soil crusts of sand dunes in Cape Cod National Seashore, Massachusetts, USA. Microb Ecol 48, 200–208.[CrossRef]
    [Google Scholar]
  29. Spatafora, J. W., Mitchell, T. G. & Vilgalys, R. ( 1995; ). Analysis of genes coding for small-subunit rRNA sequences in studying phylogenetics of dematiaceous fungal pathogens. J Clin Microbiol 33, 1322–1326.
    [Google Scholar]
  30. States, J. S. & Christensen, M. ( 2001; ). Fungi associated with biological soil crusts in the desert grasslands of Utah and Wyoming. Mycologia 93, 432–439.[CrossRef]
    [Google Scholar]
  31. Untereiner, W. A. & Naveau, F. A. ( 1999; ). Molecular systematics of the Herpotrichiellaceae with an assessment of the phylogenetic positions of Exophiala dermatididis and Phialophora americana. Mycologia 91, 67–83.[CrossRef]
    [Google Scholar]
  32. White, T. J., Bruns, T., Lee, S. & Taylor, J. W. ( 1990; ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications, pp. 315–322. Edited by M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White. New York: Academic Press.
  33. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64332-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64332-0
Loading

Data & Media loading...

Supplements

Neighbour-joining tree based on analysis of ITS sequence data showing the relationship between anam. nov. CP141b and related taxa. [PDF](22 KB)

PDF

Characteristics that differentiate strain CP141b from strain OR302-3 and nucleotides of the D1/D2 LSU nrRNA gene sequence that differentiate CP141b from other taxa in the clade. [PDF](105 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error