1887

Abstract

A high-G+C-content Gram-positive bacterium, designated as strain AC4r, was isolated from a highly alkaline, non-saline groundwater environment (pH 11.4). This organism formed small rod-shaped cells, was aerobic, heterotrophic, catalase-positive and oxidase-negative and had an optimum growth temperature of 35 °C and an optimum pH of 9.5. The strain possessed a B2-type cell-wall peptidoglycan, with -Orn as the diagnostic diamino acid. The major respiratory quinones were unsaturated menaquinones with 13 and 14 isoprene units. The predominant fatty acids were anteiso-15 : 0, iso-16 : 0, iso-14 : 0 and iso-15 : 0. The G+C content of the DNA was 67.1 mol%. In a 16S rRNA gene sequence analysis, strain AC4r showed the highest level of similarity (99.2 %) to the type strain of ; however, the DNA–DNA reassociation value between these two organisms was low (38.3 %). On the basis of phylogenetic analysis, the DNA–DNA reassociation value and distinct phenotypic characteristics, strain AC4r represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is AC4r (=LMG 22690=CIP 108473).

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/content/journal/ijsem/10.1099/ijs.0.64320-0
2006-10-01
2019-10-20
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References

  1. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  5. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  6. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  7. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  8. Nielsen, P., Fritze, D. & Priest, F. G. ( 1995; ). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745–1761.[CrossRef]
    [Google Scholar]
  9. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  10. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  11. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  12. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  13. Tiago, I., Chung, A. & Veríssimo, A. ( 2004; ). Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. Appl Environ Microbiol 70, 7378–7387.[CrossRef]
    [Google Scholar]
  14. Tiago, I., Pires, C., Mendes, V., Morais, P. V., da Costa, M. & Verissimo, A. ( 2005; ). Microcella putealis gen. nov., sp. nov., a gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 28, 479–487.[CrossRef]
    [Google Scholar]
  15. Tindall, B. J. ( 1989; ). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60, 251–254.[CrossRef]
    [Google Scholar]
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Fatty acid compositions (%) of sp. nov. AC4r and CV2 . [PDF](45 KB)

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Phylogenetic dendrogram based on a comparison of the 16S rRNA gene sequences of strain AC4r and its closest phylogenetic relatives. [PDF](13 KB)

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