1887

Abstract

In 2001, a Gram-variable, facultatively anaerobic, endospore-forming bacterium isolated from biodeteriorated mural paintings in the Servilia tomb of the Roman necropolis of Carmona was deposited as strain LMG 19508. Subsequently, the strain was characterized in detail using phenotypic and molecular methods. The 16S rRNA gene sequence confirmed that the strain belongs to the genus and indicated its relationship to CCM 4839 (96.7 % sequence similarity). The predominant menaquinone was MK-7. The cell wall contained -diaminopimelic acid of the A1 type. The DNA G+C content (50 mol%) and the major fatty acid (anteiso-C) of strain LMG 19508 were also consistent with its affiliation to the genus . DNA–DNA hybridization distinguished strain LMG 19508 from other phylogenetically related species. Therefore, the isolate represents a novel species, for which the name sp. nov. is proposed. The type strain is CCM 7311 (=LMG 19508).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64280-0
2006-10-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/10/2341.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64280-0&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64:253–260
    [Google Scholar]
  2. Dasman Kajiyama S., Kawasaki H., Yagi M., Seki T., Fukusaki E., Kobayashi A. 2002; Paenibacillus glycanilyticus sp. nov., a novel species that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune . Int J Syst Evol Microbiol 52:1669–1674 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973; The Genus Bacillus . Agriculture Handbook no: 427 Washington, DC: US Department of Agriculture;
    [Google Scholar]
  5. Heyrman J., Swings J. 2001; 16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb (necropolis of Carmona, Seville, Spain. Syst Appl Microbiol 24:417–422 [CrossRef]
    [Google Scholar]
  6. Jahnke K.-D. 1994; A modified method of quantitative colorimetric DNA-DNA hybridization on membrane filters for bacterial identification. J Microbiol Methods 20:273–288 [CrossRef]
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  8. Kanzawa Y., Harada A., Takeuchi M., Yokota A., Harada T. 1995; Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., which hydrolyse resistant curdlan. Int J Syst Bacteriol 45:515–521 [CrossRef]
    [Google Scholar]
  9. Lambert L. H., Cox T., Mitchell K., Rosselló-Mora R. A., Del Cueto C., Dodge D. E., Orkand P., Cano R. J. 1998; Staphylococcus succinus sp. nov., isolated from Dominican amber. Int J Syst Bacteriol 48:511–518 [CrossRef]
    [Google Scholar]
  10. MIDI 2001 Sherlock Microbial Identification System Operating Manual , version 4.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  11. Páčová Z., Kocur M. 1984; New medium for detection of esterase and gelatinase activity. Zentralbl Bakteriol Mikrobiol Hyg A 258:69–73
    [Google Scholar]
  12. Rivas R., Mateos P. F., Martínez-Molina E., Velázquez E. 2005; Paenibacillus phyllosphaerae sp. nov., a xylanolytic bacterium isolated from the phyllosphere of Phoenix dactylifera . Int J Syst Evol Microbiol 55:743–746 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
    [Google Scholar]
  15. Šmerda J., Sedláček I., Páčová Z., Durnová E., Smíšková A., Havel L. 2005; Paenibacillus mendelii sp. nov., a bacterium from surface-sterilized seeds of Pisum sativum L. Int J Syst Evol Microbiol 55:2351–2354 [CrossRef]
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Swarts S. G., Smith G. S., Miao L., Wheeler K. T. 1996; Effects of formic acid hydrolysis on the quantitative analysis of radiation-induced DNA base damage products assayed by gas chromatography/mass spectrometry. Radiat Environ Biophys 35:41–53 [CrossRef]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  19. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64280-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64280-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error