1887

Abstract

Two Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacteria (CCUG 46016 and CCUG 33852), isolated from a knee aspirate of a 66-year-old man and an industrial glue, respectively, were studied for their taxonomic position. On the basis of chemotaxonomic data [i.e. major ubiquinone (Q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine) and major fatty acids (C 7 and C cyclo 8)] and 16S rRNA gene sequence similarity, both strains belong to the . The presence of spermidine and putrescine as the predominant polyamines in CCUG 46016 were in agreement with its phylogenetic affiliation in the vicinity of the genus . 16S rRNA gene sequence similarities between both strains and established species within the genera , and were less than 95 %. Although both organisms showed highest 16S rRNA gene sequence similarity to members of , phenotypic features (including chemotaxonomic features) were more like those of members of the genus . Sequence comparison of the genes confirmed the separate phylogenetic position of the two strains. On the basis of DNA–DNA pairing results and physiological and biochemical data, the two strains can be clearly differentiated from each other and from all known species. It is evident that these organisms represent two novel species in a new genus, gen. nov., for which the names sp. nov. (the type species, type strain CCUG 46016=CIP 108977) and sp. nov. (type strain CCUG 33852=CIP 108976) are proposed.

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2006-08-01
2019-11-21
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Multiple sequence alignment of partial gene sequences (909 bp) of strains CCUG 46016 and CCUG 33852 with the type strains of , and and the proposed type strain of ‘ ’, with sequences of spp. and . [PDF](530 KB)

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Two-dimensional thin-layer chromatogram of polar lipids of strain CCUG 46016 . PME, Phosphatidylmonomethylethanolamine; PDE, phosphatidyldimethylethanolamine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; AL1, unknown aminolipid; PL1, unknown phospholipid, L1-5, unknown lipids.

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