1887

Abstract

The taxonomic position of a lemon-yellow-pigmented actinobacterium, strain K22-21, isolated from a soil sample from Lahaul-Spiti Valley in the Indian Himalayas, was determined using a polyphasic approach. The strain had phenotypic and chemical properties that were consistent with its classification in the genus . Alignment of the 16S rRNA gene sequence of strain K22-21 with sequences from DSM 9580, DSM 14215 and DSM 12453 revealed similarities of 98.5, 96.8 and 96.6 %, respectively. However, the level of DNA–DNA relatedness between strain K22-21 and was 55.1 %. The novel strain could be distinguished from type strains of the three species of the genus using DNA–DNA relatedness and phenotypic data. Based on these differences, strain K22-21 (=MTCC 7154=DSM 17612) should be classified as the type strain of a novel species of , for which the name sp. nov. is proposed.

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2006-08-01
2019-10-16
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References

  1. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  2. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  3. Kroppenstedt, R. M. ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationery phases. J Liq Chromatogr 5, 2359–2367.[CrossRef]
    [Google Scholar]
  4. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  5. Mayilraj, S., Prasad, G. S., Suresh, K., Saini, H. S., Shivaji, S. & Chakrabarti, T. ( 2005; ). Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55, 91–94.[CrossRef]
    [Google Scholar]
  6. Minnikin, D. E. & Goodfellow, M. ( 1976; ). Lipid composition in the classification and identification of Nocardia and related taxa. In The Biology of the Nocardiaceae, pp. 160–219. Edited by M. Goodfellow, G. H. Brownell & J. A. Serrano. London: Academic Press.
  7. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, K. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  8. Murray, R. G. E., Raymond, N., Doetsch, R. N. & Robinow, C. F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology
  9. Pandey, K. K., Mayilraj, S. & Chakrabarti, T. ( 2002; ). Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52, 1559–1567.[CrossRef]
    [Google Scholar]
  10. Park, Y. H., Suzuki, K., Yim, D. G. & 7 other authors ( 1993; ). Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie van Leeuwenhoek 64, 307–313.
    [Google Scholar]
  11. Rainey, F., Weiss, N., Prauser, H. & Stackebrandt, E. ( 1994; ). Further evidence for phylogenetic coherence of actinomycetes with group B-peptidoglycan and evidence for the phylogenetic intermixing of the genera Microbacterium and Aureobacterium as determined by 16S rDNA analysis. FEMS Microbiol Lett 118, 135–139.[CrossRef]
    [Google Scholar]
  12. Saha, P., Krishnamurthi, S., Mayilraj, S., Prasad, G. S., Bora, T. C. & Chakrabarti, T. ( 2005; ). Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. Int J Syst Evol Microbiol 55, 1491–1495.[CrossRef]
    [Google Scholar]
  13. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  14. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  15. Smith, N. R., Gordon, R. E. & Clark, F. E. ( 1952; ). Aerobic spore-forming bacteria. Agricultural Monograph no. 16. Washington, DC: US Department of Agriculture.
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  18. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  19. Suzuki, K., Sasaki, J., Uramoto, M., Nakase, T. & Komagata, K. ( 1997; ). Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate ‘Curtobacterium psychrophilum’ Inoue and Komagata 1976. Int J Syst Bacteriol 47, 474–478.[CrossRef]
    [Google Scholar]
  20. Tourova, T. P. & Antonov, A. S. ( 1987; ). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19, 333–355.
    [Google Scholar]
  21. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  23. Wieser, M., Schumann, P., Martin, K., Altenburger, P., Burghardt, J., Lubitz, W. & Busse, H.-J. ( 1999; ). Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Bacteriol 49, 1165–1170.[CrossRef]
    [Google Scholar]
  24. Zlamala, C., Schumann, P., Kämpfer, P., Rosselló-Mora, R., Lubitz, W. & Busse, H.-J. ( 2002; ). Agrococcus baldri sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52, 1211–1216.[CrossRef]
    [Google Scholar]
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vol. , part 8, pp. 1807 - 1810

Fatty acid profiles of strain K22-21 and related type strains. [PDF](15 KB)



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