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Abstract

Bacteria-like endosymbionts of females of the plant-parasitic nematodes and and juveniles of were first observed during transmission electron microscopy (TEM) studies conducted in the 1970s. These organisms were characterized as being rod-shaped, ranging in size from 0.3 to 0.5 μm in diameter and 1.8 to 3 μm in length and containing structures labelled as striated inclusion bodies or tubular structures. A population of was obtained from the soybean field where infected nematodes were first discovered in order to conduct TEM studies of females and males and to determine the phylogenetic position of the endosymbiont among bacteria by studying the 16S rRNA and gene sequences. The bacterium was observed in the pseudocoelom and intestine of juveniles, females and males, in hypodermal chords of juveniles and males, in ovary walls and in oocytes and spermatozoa. The bacterium was polymorphic, measuring 0.4–0.8×2.5–4.5 μm, and many specimens contained an array of microfilament-like structures similar to those observed in ‘ Cardinium hertigii’, the endosymbiont of spp. wasps. Phylogenetic analysis of the 16S rRNA and genes of the -infecting bacterium revealed 93 % and 81 % sequence identity, respectively, to the homologous genes in ‘ C. hertigii’. Thus, the name ‘ Paenicardinium endonii’ is proposed for the bacterial endosymbiont of the plant-parasitic nematode .

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2006-07-01
2019-10-17
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vol. , part 7, pp. 1697 - 1702

Phylogenetic position of the bacterial endosymbiont of , ‘ Paenicardinium endonii’, inferred from maximum-likelihood analysis of 16S rRNA gene sequences from selected members of the phylum .

Phylogenetic position of ‘ Paenicardinium endonii’ inferred from maximum-likelihood analysis of gene sequences from selected members of the phylum .

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