Two Gram-positive bacilli, designated as strains SMC 4352-1 and SMC 4352-2, were isolated sequentially from the blood of a newborn child with sepsis. They could not be identified by using conventional clinical microbiological methods. 16S rRNA gene sequencing and phylogenetic analysis revealed that both strains belonged to the genus but clearly diverged from known species. Strain SMC 4352-1 and strain SMC 4352-2 were found to be closely related to NCIMB 9366 (98.2 % sequence similarity) and JG-30 (97.1 % sequence similarity), respectively. They also displayed low DNA–DNA reassociation values (less than 40 %) with respect to the most closely related species. On the basis of their polyphasic characteristics, strain SMC 4352-1 and strain SMC 4352-2 represent two novel species of the genus , for which the names sp. nov. (type strain SMC 4352-1=KCCM 90025=JCM 13438) and sp. nov. (type strain SMC 4352-2=KCCM 90024=JCM 13437) are proposed.


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vol. , part 11, pp. 2541 - 2544

Fatty acid compositions of the two novel strains. [PDF](43 KB)


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