1887

Abstract

A coral-red-pigmented actinobacterium, strain K30-10, was isolated from a soil sample from a cold desert of the Indian Himalayas. Chemical and phenotypic properties of strain K30-10 were consistent with its classification in the genus . It showed 97.9 % 16S rRNA gene sequence similarity to MTCC 7011; similarities to the type strains of three other species of the genus, , and , were 94.4–96.0 %. The DNA–DNA relatedness between K30-10 and the closely related strain MTCC 7011 was 59.2 %. The DNA G+C content of strain K30-10 was 67.0 mol%. Based on physiological and biochemical tests and genotypic differences between strain K30-10 and its closest phylogenetic relatives, it is proposed that this strain represents a novel species, sp. nov.; the type strain is K30-10 (=MTCC 7007=DSM 44907=JCM 13325).

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2006-07-01
2019-10-21
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References

  1. Colquhoun, J. A., Heald, S. C., Li, L., Tamaoka, J., Kato, C., Horikoshi, K. & Bull, A. T. ( 1998; ). Taxonomy and biotransformation activities of some deep-sea actinomycetes. Extremophiles 2, 269–277.[CrossRef]
    [Google Scholar]
  2. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  3. Duckworth, A. W., Grant, S., Grant, W. D., Jones, B. E. & Meijer, D. ( 1998; ). Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake. Extremophiles 2, 359–366.[CrossRef]
    [Google Scholar]
  4. Klatte, S., Rainey, F. A. & Kroppenstedt, R. M. ( 1994; ). Transfer of Rhodococcus aichiensis Tsukamura 1982 and Nocardia amarae Lechevalier and Lechevalier 1974 to the genus Gordona as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int J Syst Bacteriol 44, 769–773.[CrossRef]
    [Google Scholar]
  5. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  6. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance–temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  7. Mayilraj, S., Prasad, G. S., Suresh, K., Saini, H. S., Shivaji, S. & Chakrabarti, T. ( 2005; ). Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55, 91–94.[CrossRef]
    [Google Scholar]
  8. Minnikin, D. E. & Goodfellow, M. ( 1976; ). Lipid composition in the classification and identification of Nocardia and related taxa. In The Biology of the Nocardiaceae, pp. 160–219. Edited by M. Goodfellow, G. H. Brownell & J. A. Serrano. London: Academic Press.
  9. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, K. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  10. Murray, R. G. E., Raymond, N., Doetsch, R. N. & Robinow, C. F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  11. Pandey, K. K., Mayilraj, S. & Chakrabarti, T. ( 2002; ). Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52, 1559–1567.[CrossRef]
    [Google Scholar]
  12. Rainey, F. A., Klatte, S., Kroppenstedt, R. M. & Stackebrandt, E. ( 1995; ). Dietzia, a new genus including Dietzia maris comb. nov., formerly Rhodococcus maris. Int J Syst Bacteriol 45, 32–36.[CrossRef]
    [Google Scholar]
  13. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  14. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  15. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  16. Tourova, T. P. & Antonov, A. S. ( 1987; ). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19, 333–355.
    [Google Scholar]
  17. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  18. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  19. Yassin, A. F., Hupfer, H. & Schaal, K. P. ( 2006; ). Dietzia cinnamea sp. nov., a novel species isolated from a perianal swab of a patient with a bone marrow transplant. Int J Syst Evol Microbiol 56, 641–645.[CrossRef]
    [Google Scholar]
  20. Yumoto, I., Nakamura, A., Iwata, H., Kojima, K., Kusumoto, K., Nodasaka, Y. & Matsuyama, H. ( 2002; ). Dietzia psychralcaliphila sp. nov., a novel, facultatively psychrophilic alkaliphile that grows on hydrocarbons. Int J Syst Evol Microbiol 52, 85–90.
    [Google Scholar]
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