1887

Abstract

In the last few years, many attempts have been made to use conserved gene sequences for identification and for phylogenetic studies of species. In an effort to identify a more reliable approach, a gene sequence-based database was created. In this study, an approximately 883 bp portion of the gene sequence from 45 staphylococcal type strains was compared with 16S rRNA and other conserved gene (, and ) sequences available in public databases. Nucleotide sequence comparisons revealed that the staphylococcal gene showed higher discrimination (mean similarity 77.6 %) than the 16S rRNA (mean similarity 97.4 %), (mean similarity 86 %), (mean similarity 82 %) and (mean similarity 81.5 %) genes. Analysis of the gene sequence from 20 isolates representing two clinically important species showed <1 % sequence divergence. Phylogenetic data obtained from the gene sequence were in general agreement with those of 16S rRNA gene sequence analysis and DNA–DNA reassociation studies. In conclusion, the gene sequence-based assay is an effective alternative to currently used methods, including 16S rRNA gene sequencing, for identification and taxonomical analysis of species.

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2007-01-01
2019-10-18
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vol. , part 1, pp. 25 - 30

Sequence similarity percentage based on pairwise comparisons of and 16S rRNA gene sequences of species and subspecies. [PDF](657 KB)



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