1887

Abstract

A Gram-positive, rod-shaped, non-endospore-forming but mycelium-forming actinobacterium (strain K1) was isolated from an enrichment culture containing tetrahydrofuran (THF) as the sole source of carbon. On the basis of its G+C content (71.3 mol%) and of 16S rRNA gene sequence similarity studies, strain K1 was shown to belong to the family , most closely related to (99.3 %), (98.8 %) and (98.3 %). The 16S rRNA gene sequence similarity to other species was less than 97 %. Chemotaxonomic data [major menaquinone MK-8(H); major fatty acids C iso, C iso and C 6] supported the affiliation of strain K1 to the genus . The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain K1 from the three species , and , although all four organisms utilized THF. Strain K1 represents a novel species, for which the name sp. nov. is proposed, with the type strain K1 (=DSM 44239=CIP 109050).

Keyword(s): THF, tetrahydrofuran
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2006-07-01
2019-10-15
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Phylogenetic analysis based on 16S rRNA gene sequences of strain K1 and related sequences available from the EMBL database, constructed after multiple alignment of data by CLUSTAL X, with distances determined according to the Kimura-2 model and clustering with the maximum-parsimony method. [PDF](42 KB)

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Major fatty acids of strain K1 and type strains of closely related species of the genus . [PDF](24 KB)

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